ACE2 Protein (Fc Tag,AVI tag,Biotin)
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- 抗原 See all ACE2 蛋白
- ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This ACE2 protein is labelled with Fc Tag,AVI tag,Biotin.
- 序列
- AA 18-740
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 产品特性
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Biotinylated Human ACE2, Fc,Avitag is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1). Predicted N-terminus: Gln 18
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). - 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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- Discover our top product ACE2 蛋白
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Angiotensin I Converting Enzyme 2 (ACE2) protein (His tag,AVI tag,Biotin)
ACE2 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. SDS, Func
Angiotensin I Converting Enzyme 2 (ACE2) protein (His tag)ACE2 宿主: Paguma larvata 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. SDS
Angiotensin I Converting Enzyme 2 (ACE2) (AA 18-740) protein (Fc Tag)ACE2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Angiotensin I Converting Enzyme 2 (ACE2) protein (His-Avi Tag,Biotin)ACE2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Angiotensin I Converting Enzyme 2 (ACE2) protein (His-Avi Tag)ACE2 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Angiotensin I Converting Enzyme 2 (ACE2) protein (His-Avi Tag)ACE2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Angiotensin I Converting Enzyme 2 (ACE2) (AA 18-740) protein (His tag)ACE2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Angiotensin I Converting Enzyme 2 (ACE2) (AA 18-740) protein (Fc-Avi Tag,Biotin)ACE2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Angiotensin I Converting Enzyme 2 (ACE2) protein (mFc Tag)ACE2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Angiotensin I Converting Enzyme 2 (ACE2) protein (His-Avi Tag,Biotin)ACE2 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
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- 应用备注
- The protein has a calculated MW of 111.7 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.
- 说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 50 mM Tris, 150 mM NaCl, pH 7.5
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
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The product MUST be stored at -70°C or lower upon receipt
-70°C for 3 months under sterile conditions.
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: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." in: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).
: "Dromedary camels as a natural source of neutralizing nanobodies against SARS-CoV-2." in: JCI insight, (2021) (PubMed).
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: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." in: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).
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- 抗原
- ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
- 别名
- ACE2 (ACE2 产品)
- 别名
- ACE1 Protein, CD143 Protein, DCP Protein, DCP1 Protein, ICH Protein, MVCD3 Protein, ACEH Protein, 2010305L05Rik Protein, zgc:92514 Protein, angiotensin I converting enzyme Protein, angiotensin I converting enzyme 2 Protein, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 Protein, ACE Protein, ACE2 Protein, Ace2 Protein, ace2 Protein
- 背景
- Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.
- 分子量
- 111.7 kDa
- NCBI登录号
- NP_068576
- 途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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