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ACE2 Protein (His tag,AVI tag,Biotin)

ACE2 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. SDS, Func
产品编号 ABIN6952431
发货至: 中国
  • 抗原 See all ACE2 蛋白
    ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
    蛋白类型
    Recombinant
    宿主
    • 44
    • 7
    • 6
    • 3
    • 2
    • 1
    • 1
    资源
    • 30
    • 11
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    • 1
    HEK-293 Cells
    标记
    This ACE2 protein is labelled with His tag,AVI tag,Biotin.
    应用范围
    SDS-PAGE (SDS), Functional Studies (Func)
    品牌
    MABSol®,PrecisionAvi
    序列
    AA 18-740
    特异性
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    产品特性
    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 87.2 kDa. The protein migrates as 95-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    纯度
    >90 % as determined by SDS-PAGE.
    内毒素水平
    Less than 1.0 EU per μg by the LAL method.
  • 说明

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    50 mM Tris, 150 mM NaCl, pH 7.5
    注意事项
    Please avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    -20°C
  • Bell, Powell, Rodriguez, Cochran, Kim: "Neutralizing antibodies targeting the SARS-CoV-2 receptor binding domain isolated from a naïve human antibody library." in: Protein science : a publication of the Protein Society, Vol. 30, Issue 4, pp. 716-727, (2021) (PubMed).

  • 抗原
    ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
    别名
    ACE2 (ACE2 产品)
    别名
    ACE1 Protein, CD143 Protein, DCP Protein, DCP1 Protein, ICH Protein, MVCD3 Protein, ACEH Protein, 2010305L05Rik Protein, zgc:92514 Protein, angiotensin I converting enzyme Protein, angiotensin I converting enzyme 2 Protein, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 Protein, ACE Protein, ACE2 Protein, Ace2 Protein, ace2 Protein
    背景
    Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.
    分子量
    87.2 kDa
    NCBI登录号
    NP_068576
    途径
    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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