电话:: 400-7060-959
传真:: +86 10 56315212-8813
电子邮件:: orders@antibodies-online.cn

我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

SARS-CoV-2 Spike S1 Protein (His tag)

宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. SDS, Func

Immobilized SARS-CoV-2 S1 protein, His Tag (ABIN6952427,ABIN6952430) at 2 μg/mL (100 μL/well) can bind Monoclonal A-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.

2019-nCoV S1 protein, His Tag on SDS-PAGE under reducing (R) condition.

Immobilized 2019-nCoV S1 protein, His Tag ( ABIN6952427) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 2-20 ng/mL (QC tested).

Human ACE2, Fc Tag ( ABIN6952431) Captured on CM5 chip via anti-human IgG Fc antibodies surface can bind 2019-nCoV S1 protein, His Tag ( ABIN6952427) with an affinity constant of 64.

2019-nCoV S1 protein, His Tag ( ABIN6952427) captured on CM5 Chip via anti-His antibody can bind Human ACE2, Fc Tag ( ABIN6952431) with an affinity constant of 15.

Loaded Human ACE2, Fc Tag ( ABIN6952431) on Protein A Biosensor, can bind 2019-nCoV S1 protein, His Tag ( ABIN6952427) with an affinity constant of 19.

6 images

(9 references)

产品编号 ABIN6952427

发货至: 中国

Datasheet as PDF

抗原 See all SARS-CoV-2 Spike S1 蛋白

SARS-CoV-2 Spike S1

蛋白类型

Recombinant
宿主
94

9

5

3

2

2

2

1
SARS Coronavirus-2 (SARS-CoV-2)
资源
86

6

3

3

1

1

1

1

1
HEK-293 Cells

标记

This SARS-CoV-2 Spike S1 protein is labelled with His tag.

应用范围

SDS-PAGE (SDS), Functional Studies (Func)

序列

AA 16-685

产品特性

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76.9 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
AA 16-685

纯度

>90 % as determined by SDS-PAGE.

内毒素水平

Less than 1.0 EU per μg by the LAL method.

Biological Activity Comment

Immobilized 2019-nCoV S1 protein, His Tag at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 2-20 ng/mL (QC tested).
SARS-CoV-2 Spike S1 (RBD) protein (His-SUMOstar Tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 酵母菌 Recombinant Greater than 85 % as determined by SDS-PAGE.

SARS-CoV-2 Spike S1 (RBD) protein (His tag,MYC tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: Mammalian Cells Recombinant Greater than 90 % as determined by SDS-PAGE.

SARS-CoV-2 Spike S1 (B.1.1.529 - Omicron) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

SARS-CoV-2 Spike S1 (RBD) protein (His tag,AVI tag,Biotin)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.

SARS-CoV-2 Spike S1 (BA.4 - Omicron), (BA.5 - Omicron) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

SARS-CoV-2 Spike S1 (BA.2.12.1 - Omicron) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

SARS-CoV-2 Spike S1 protein (Fc-Avi Tag)
宿主: SARS Coronavirus (SARS-CoV) 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

SARS-CoV-2 Spike S1 (N501Y) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

SARS-CoV-2 Spike S1 (E484K), (K417N), (N501Y) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

SARS-CoV-2 Spike S1 (C.37 - Lambda) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4

注意事项

Please avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

-20°C
Fan, Parr, Kang, Gupta: "Point-of-care (POC) SARS-CoV-2 antigen detection using functionalized aerosol jet-printed organic electrochemical transistors (OECTs)." in: Nanoscale, Vol. 15, Issue 11, pp. 5476-5485, (2023) (PubMed).

Lucas, Klein, Sundaram, Liu, Wong, Silva, Mao, Oh, Mohanty, Huang, Tokuyama, Lu, Venkataraman, Park, Israelow, Vogels, Muenker, Chang, Casanovas-Massana, Moore, Zell, Fournier, Wyllie, Campbell, Lee et al.: "Delayed production of neutralizing antibodies correlates with fatal COVID-19. ..." in: Nature medicine, (2021) (PubMed).

Song, Bartley, Chow, Ngo, Jiang, Zamecnik, Dandekar, Loudermilk, Dai, Liu, Sunshine, Liu, Wu, Hawes, Alvarenga, Huynh, McAlpine, Rahman, Geng, Chiarella, Goldman-Israelow, Vogels, Grubaugh et al.: "Divergent and self-reactive immune responses in the CNS of COVID-19 patients with neurological symptoms. ..." in: Cell reports. Medicine, pp. 100288, (2021) (PubMed).

Maritz, Woudberg, Bennett, Soares, Lapierre, Devine, Kimberg, Bouic: "Validation of high-throughput, semiquantitative solid phase SARS coronavirus-2 serology assays in serum and dried blood spot matrices." in: Bioanalysis, (2021) (PubMed).

Mazzini, Martinuzzi, Hyseni, Benincasa, Molesti, Casa, Lapini, Piu, Trombetta, Marchi, Razzano, Manenti, Montomoli: "Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples." in: Journal of immunological methods, Vol. 489, pp. 112937, (2021) (PubMed).

Haslwanter, Dieterle, Wec, OBrien, Sakharkar, Florez, Tong, Rappazzo, Lasso, Vergnolle, Wirchnianski, Bortz, Laudermilch, Fels, Mengotto, Malonis, Georgiev, Quiroz, Wrapp, Wang, Dye, Barnhill, Dye et al.: "A Combination of Receptor-Binding Domain and N-Terminal Domain Neutralizing Antibodies Limits the Generation of SARS-CoV-2 Spike Neutralization-Escape Mutants. ..." in: mBio, Vol. 12, Issue 5, pp. e0247321, (2021) (PubMed).

Pierce, Preston-Hurlburt, Dai, Aschner, Cheshenko, Galen, Garforth, Herrera, Jangra, Morano, Orner, Sy, Chandran, Dziura, Almo, Ring, Keller, Herold, Herold: "Immune responses to SARS-CoV-2 infection in hospitalized pediatric and adult patients." in: Science translational medicine, Vol. 12, Issue 564, (2020) (PubMed).

Luetkens, Metcalf, Planelles, Zheng, Larragoite, Spivak, Spivak, Steinbach, Blaylock, Avila, Hankey, Martins, Slev, Mannuel, Sajadi, Rapoport, Atanackovic: "Successful transfer of anti-SARS-CoV-2 immunity using convalescent plasma in an MM patient with hypogammaglobulinemia and COVID-19." in: Blood advances, Vol. 4, Issue 19, pp. 4864-4868, (2020) (PubMed).

Wei, Wan, Yan, Wang, Zhang, Yang, Zhang, Fan, Li, Deng, Sun, Gong, Yang, Wang, Wang, Li, Yang, Li, Zhang, Wang, Du, Zong, Yin, Zhang, Wang, Peng, Lin, Feng, Qin, Chen, Gao, Zhang, Cao, Zhong: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." in: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, (2020) (PubMed).
抗原

SARS-CoV-2 Spike S1

Abstract

SARS-CoV-2 Spike S1 产品

别名

E2 Protein, Surface Glycoprotein Protein, S Protein

物质类

Viral Protein

背景

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

分子量

76.9 kDa

基因ID

43740568

UniProt

P0DTC2

You are here: