SEMA3A Protein (AA 31-141) (His tag)
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- 抗原 See all SEMA3A 蛋白
- SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))
- 生物活性
- Active
- 产品特性
- AA 31-141
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This SEMA3A protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- The Semaphorin 3A (SEMA3A) which belongs to the semaphorin family can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Semaphorin 3A is secreted protein containing a Sema domain, an immunoglobulin C2-like domain and a basic domain near the carboxyl tail. It can be secreted by neurons and surrounding tissue to guide migrating cells and axons in the developing nervous system. Besides, Neuropilin 1 (NRP1) has been identified as an interactor of SEMA3A, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SEMA3A and recombinant human NRP1. Briefly, SEMA3A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to NRP1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-SEMA3A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of SEMA3A and NRP1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of SEMA3A with NRP1.
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- Discover our top product SEMA3A 蛋白
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Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A) (AA 21-772) protein (His tag)
SEMA3A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A) (AA 672-770) protein (GST tag)SEMA3A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A) protein (Fc Tag)SEMA3A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE
Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A) (AA 21-771) protein (His tag)Crystallography grade SEMA3A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A) (AA 21-772) protein (His tag)Crystallography grade SEMA3A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Isoelectric Point: 8.9
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))
- 别名
- Semaphorin 3A (SEMA3A) (SEMA3A 产品)
- 别名
- COLL1 Protein, HH16 Protein, Hsema-I Protein, Hsema-III Protein, SEMA1 Protein, SEMAD Protein, SEMAIII Protein, SEMAL Protein, SemD Protein, coll-1 Protein, Semad Protein, COLL-1 Protein, SEMA3A Protein, Dsema3a Protein, Sema3A Protein, sema1a Protein, sema3a1 Protein, semaZ1a Protein, sema1b Protein, sema3a2 Protein, semaZ1b Protein, semaphorin 3A Protein, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A Protein, Sema-3a Protein, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Aa Protein, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Ab Protein, SEMA3A Protein, Sema3a Protein, Sema-3a Protein, sema3aa Protein, sema3ab Protein
- 背景
- Alternative Names: SEMAL, Hsema-I, Hsema-III, SEMA1, SEMAD, SEMAIII, SemD, Coll-1, Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A
- 分子量
- 16kDa
- UniProt
- Q14563
- 途径
- Regulation of Cell Size
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