MFAP2 Protein (AA 6-162) (His tag)
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- 抗原 See all MFAP2 蛋白
- MFAP2 (Microfibrillar Associated Protein 2 (MFAP2))
- 生物活性
- Active
- 产品特性
- AA 6-162
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This MFAP2 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- Microfibrillar-associated protein 2 (MFAP2) is an O-glycosylated protein which excreted to the extracellular space and the extracellular matrix. MFAP2 combine biglycan and elastin to form a ternary complex. MFAP2 plays a key role in the support and distensibility of the juxtacanalicular region of these collector channels. It also can inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Besides, Fibrillin 1 (FBN1) has been identified as an interactor of MFAP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MFAP2 and recombinant human FBN1. Briefly, MFAP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FBN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MFAP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of MFAP2 and FBN1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of MFAP2 with FBN1.
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- 应用备注
- Isoelectric Point: 5.5
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- MFAP2 (Microfibrillar Associated Protein 2 (MFAP2))
- Abstract
- MFAP2 产品
- 别名
- MAGP Protein, MAGP-1 Protein, MAGP1 Protein, AI893631 Protein, Magp Protein, Magp1 Protein, microfibril associated protein 2 Protein, microfibrillar-associated protein 2 Protein, MFAP2 Protein, Mfap2 Protein
- 背景
- Alternative Names: MAGP1, Microfibril-associated glycoprotein 1
- 分子量
- 38kDa
- UniProt
- P55001
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