PON3 Protein (AA 2-354) (His tag)
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- 抗原 See all PON3 蛋白
- PON3 (Paraoxonase 3 (PON3))
- 生物活性
- Active
- 产品特性
- AA 2-354
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PON3 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Paraoxonase 3 (PON3) is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 also rapidly hydrolyzes lactones and can inhibit the oxidation of low-density lipoprotein, a function that is believed to slow the initiation and progression of atherosclerosis. Besides, Paraoxonase 1 (PON1) has been identified as an interactor of PON3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON3 and recombinant human PON1. Briefly, PON3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PON1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PON3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of PON3 and PON1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of PON3 with PON1.
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Paraoxonase 3 (PON3) (AA 1-354) protein (GST tag)
PON3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Paraoxonase 3 (PON3) (AA 2-354) protein (His tag)Crystallography grade PON3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Isoelectric Point: 5.2
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- PON3 (Paraoxonase 3 (PON3))
- Abstract
- PON3 产品
- 别名
- 2810004E20 Protein, AI786302 Protein, paraoxonase 3 Protein, PON3 Protein, Pon3 Protein
- 背景
- Alternative Names: Serum paraoxonase/lactonase 3
- 分子量
- 43kDa
- UniProt
- Q15166
- 途径
- Monocarboxylic Acid Catabolic Process
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