APRT Protein (AA 1-180) (His tag)
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- 抗原 See all APRT 蛋白
- APRT (Adenine Phosphoribosyltransferase (APRT))
- 生物活性
- Active
- 产品特性
- AA 1-180
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This APRT protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Adenine Phosphoribosyltransferase (APRT) is an enzyme involved in the purine nucleotide salvage pathway. It functions as a catalyst in the reaction between adenine and phosphoribosyl pyrophosphate (PRPP) to form AMP. Besides, Vascular Cell Adhesion Molecule 1 (VCAM1) has been identified as an interactor of APRT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APRT and recombinant human VCAM1. Briefly, APRT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to VCAM1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-APRT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of APRT and VCAM1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of APRT with VCAM1.
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Adenine Phosphoribosyltransferase (APRT) (AA 1-180) protein (GST tag)
APRT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Adenine Phosphoribosyltransferase (APRT) (AA 2-180) protein (His tag)Crystallography grade APRT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Adenine Phosphoribosyltransferase (APRT) (AA 2-180) protein (His tag)Crystallography grade APRT 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Adenine Phosphoribosyltransferase (APRT) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)APRT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Isoelectric Point: 6.8
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- APRT (Adenine Phosphoribosyltransferase (APRT))
- Abstract
- APRT 产品
- 别名
- Tb07.43M14.200 Protein, Tb07.43M14.180 Protein, C85684 Protein, AMP Protein, APRTD Protein, adenine phosphoribosyltransferase Protein, adenine phosphoribosyl transferase Protein, CND05020 Protein, Tb927.7.1790 Protein, Tb927.7.1780 Protein, Arnit_0941 Protein, Saut_1231 Protein, Fbal_1180 Protein, PH_RS07970 Protein, PF_RS08760 Protein, PAB_RS02560 Protein, Aprt Protein, APRT Protein
- 背景
- Alternative Names: AMP, APRTase
- 分子量
- 23kDa
- UniProt
- P07741
- 途径
- Ribonucleoside Biosynthetic Process
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