CAPNL1 Protein (AA 387-694) (His tag)
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- 抗原 See all CAPNL1 蛋白
- CAPNL1 (Calpain 1, Large Subunit (CAPNL1))
- 生物活性
- Active
- 产品特性
- AA 387-694
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CAPNL1 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CAPN1 with STAT3.
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- Discover our top product CAPNL1 蛋白
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Calpain 1, Large Subunit (CAPNL1) (AA 355-526) protein (His-IF2DI Tag)
CAPNL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses ELISA, WB
Calpain 1, Large Subunit (CAPNL1) (AA 610-714) protein (GST tag)CAPNL1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Calpain 1, Large Subunit (CAPNL1) (AA 2-714) protein (His tag)Crystallography grade CAPNL1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Calpain 1, Large Subunit (CAPNL1) (AA 2-713) protein (His tag)Crystallography grade CAPNL1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Calpain 1, Large Subunit (CAPNL1) (AA 75-356) (Active) protein (His tag)CAPNL1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Calpain 1, Large Subunit (CAPNL1) proteinCAPNL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % ELISA, WB, SDS, IP
Calpain 1, Large Subunit (CAPNL1) proteinCAPNL1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % ELISA, WB, SDS, IP
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- 应用备注
- Isoelectric Point: 5.9
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- CAPNL1 (Calpain 1, Large Subunit (CAPNL1))
- 别名
- Calpain 1, Large Subunit (CAPN1) (CAPNL1 产品)
- 别名
- CANP Protein, CANP1 Protein, CANPL1 Protein, muCANP Protein, muCL Protein, Capa-1 Protein, Capa1 Protein, mu-calpin Protein, si:ch211-203k12.4 Protein, si:dkeyp-101g1.3 Protein, calpain Protein, calpain 1 Protein, calpain 1, (mu/I) large subunit Protein, calpain 1, (mu/I) large subunit b Protein, calpain 11 Protein, CAPN1 Protein, Capn1 Protein, capn1 Protein, capn1b Protein, CAPN11 Protein
- 背景
- Alternative Names: PIG30, CANPL1, MuCANP, MuCL, Calpain-1 Catalytic Subunit, Calcium-Dependent Protease 1, Calcium-activated neutral proteinase 1, Calpain mu-type, Micromolar-calpain
- 分子量
- 35kDa
- UniProt
- P97571
- 途径
- Apoptosis
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