GOLM1 Protein (AA 133-379) (His tag,GST tag)
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- 抗原 See all GOLM1 蛋白
- GOLM1 (Golgi Membrane Protein 1 (GOLM1))
- 生物活性
- Active
- 产品特性
- AA 133-379
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This GOLM1 protein is labelled with His tag,GST tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His and GST Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Golgi Protein 73 (GP73) also known as Golgi phosphoprotein 2 or Golgi membrane protein GP73 is a protein that encoded by the GOLM1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. It has been observed to be upregulated in response to viral infection. Besides, Dymeclin (DYM) has been identified as an interactor of GP73, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse GP73 and recombinant human DYM. Briefly, GP73 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to DYM-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GP73 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GP73 and DYM was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of GP73 with DYM.
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Golgi Membrane Protein 1 (GOLM1) (AA 101-401) protein (His tag)
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Golgi Membrane Protein 1 (GOLM1) (AA 36-393) protein (His tag)Crystallography grade GOLM1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Golgi Membrane Protein 1 (GOLM1) (AA 1-393) protein (Strep Tag)Crystallography grade GOLM1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- 应用备注
- Isoelectric Point: 5.2
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- GOLM1 (Golgi Membrane Protein 1 (GOLM1))
- 别名
- Golgi Protein 73 (GP73) (GOLM1 产品)
- 别名
- C9orf155 Protein, GOLPH2 Protein, GP73 Protein, PSEC0257 Protein, bA379P1.3 Protein, 2310001L02Rik Protein, AW125446 Protein, D030064E01Rik Protein, Golph2 Protein, GOLM1 Protein, golph2 Protein, gp73 Protein, golgi membrane protein 1 Protein, SPATA31 subfamily D, member 3 Protein, Golgi membrane protein 1 Protein, golgi membrane protein 1 L homeolog Protein, GOLM1 Protein, Golm1 Protein, Spata31d3 Protein, golm1 Protein, LOC100400549 Protein, golm1.L Protein
- 背景
- Alternative Names: GOLM1, GOLPH2, C9orf155, Golgi Membrane Protein 1, Golgi Phosphoprotein 2
- 分子量
- 58kDa
- UniProt
- Q91XA2
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