CYP1A2 Protein (AA 2-231) (His tag,GST tag)
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- 抗原 See all CYP1A2 蛋白
- CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))
- 生物活性
- Active
- 产品特性
- AA 2-231
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CYP1A2 protein is labelled with His tag,GST tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His and GST Tag
- 纯度
- > 95 %
- Biological Activity Comment
- CYP1A2 (Cytochrome P450 1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Besides, ASAH1 (Acid ceramidase) has been identified as an interactor of CYP1A2 through affinity capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ASAH1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CYP1A2 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1A2 and ASAH1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CYP1A2 with ASAH1.
- Top Product
- Discover our top product CYP1A2 蛋白
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Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2) (AA 211-310) protein (GST tag)
CYP1A2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2) (AA 1-516) protein (GST tag)CYP1A2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2) (AA 2-515) protein (His tag)Crystallography grade CYP1A2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2) (AA 2-513) protein (His tag)Crystallography grade CYP1A2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2) protein (Myc-DYKDDDDK Tag)CYP1A2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Isoelectric Point: 8
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))
- 别名
- Cytochrome P450 1A2 (CYP1A2) (CYP1A2 产品)
- 别名
- CP12 Protein, P3-450 Protein, P450(PA) Protein, CYP1A2 Protein, CYPIA2 Protein, P450-PM4 Protein, Cyp1a1 Protein, P450-3 Protein, CYPD45 Protein, P-450d Protein, RATCYPD45 Protein, CYP1A5 Protein, CP450 Protein, CYP-IA2 Protein, cytochrome P450 family 1 subfamily A member 2 Protein, cytochrome P450 1A2 Protein, cytochrome P450 LM4 Protein, cytochrome P450, family 1, subfamily A, polypeptide 2 Protein, cytochrome P450, family 1, subfamily a, polypeptide 2 Protein, cytochrome P450 1A2-like Protein, CYP1A2 Protein, LOC100328937 Protein, Cyp1a2 Protein, VDBG_06455 Protein, LOC101825565 Protein, LOC102130662 Protein
- 背景
- Alternative Names: CP12, P3-450, P450(PA), Cytochrome P450,Family 1,Subfamily A,Polypeptide 2
- 分子量
- 55kDa
- UniProt
- P05177
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