NEU1 Protein (AA 141-286) (His tag)
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- 抗原 See all NEU1 蛋白
- NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))
- 生物活性
- Active
- 产品特性
- AA 141-286
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This NEU1 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of NEU with CTSA.
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Sialidase 1 (Lysosomal Sialidase) (NEU1) (AA 48-415) protein (His tag)
NEU1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Sialidase 1 (Lysosomal Sialidase) (NEU1) (AA 334-415) protein (GST tag)NEU1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sialidase 1 (Lysosomal Sialidase) (NEU1) (AA 48-415) protein (His tag)Crystallography grade NEU1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Sialidase 1 (Lysosomal Sialidase) (NEU1) (AA 42-409) protein (His tag)Crystallography grade NEU1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Sialidase 1 (Lysosomal Sialidase) (NEU1) (AA 47-415) (Active) protein (His tag)NEU1 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
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- 应用备注
- Isoelectric Point: 5.5
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))
- 别名
- Neuraminidase (NEU) (NEU1 产品)
- 别名
- NANH Protein, NEU Protein, SIAL1 Protein, MGC81958 Protein, AA407268 Protein, AA407316 Protein, Aglp Protein, Apl Protein, Bat-7 Protein, Bat7 Protein, G9 Protein, Map-2 Protein, Neu Protein, Neu-1 Protein, neuraminidase 1 Protein, neuraminidase 1 (lysosomal sialidase) L homeolog Protein, NEU1 Protein, neu1.L Protein, neu1 Protein, Neu1 Protein
- 物质类
- Influenza Protein
- 背景
- Alternative Names: NEU1, SIAL1, Sialidase 1, Lysosomal Sialidase, Acetylneuraminyl hydrolase, G9 sialidase, N-acetyl-alpha-neuraminidase 1
- 分子量
- 26kDa
- UniProt
- Q99519
- 途径
- SARS-CoV-2 Protein Interactome
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