PDGFA Protein (AA 94-194) (His tag)
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- 抗原 See all PDGFA 蛋白
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
- 生物活性
- Active
- 产品特性
- AA 94-194
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PDGFA protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- PDGFA (Platelet-derived growth factor subunit A) is a Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA has been described as a chemoattractant for monocytes and proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of PDGFA on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and PDGFA (1.5ng/mL, 7.5ng/mL and 15ng/mL diluted separately in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37oC with 5%CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x40) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). Result shows PDGFA is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (x40) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (x400). Statistical results were shown in Figure 2. The optimum chemotaxis of PDGFA occurs at 1.5ng/mL.
- Top Product
- Discover our top product PDGFA 蛋白
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Platelet Derived Growth Factor A (PDGFA) (Active) protein
PDGFA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
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Platelet Derived Growth Factor A (PDGFA) (Active) proteinPDGFA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Platelet Derived Growth Factor A (PDGFA) (AA 84-193) protein (GST tag)PDGFA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Platelet Derived Growth Factor A (PDGFA) (AA 87-211) protein (His tag)Crystallography grade PDGFA 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Platelet Derived Growth Factor A (PDGFA) (AA 87-211) protein (His tag)Crystallography grade PDGFA 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Isoelectric Point: 8.9
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
- 别名
- Platelet Derived Growth Factor Subunit A (PDGFA) (PDGFA 产品)
- 别名
- PDGF-A Protein, PDGF1 Protein, pdgfa Protein, pdgfa-2 Protein, zgc:100777 Protein, pdgf Protein, pdgf1 Protein, pdgf-a Protein, AA409063 Protein, CD142 Protein, Cf-3 Protein, Cf3 Protein, TF Protein, PDGFACP Protein, wu:fc32c12 Protein, zgc:153249 Protein, platelet derived growth factor subunit A Protein, platelet-derived growth factor alpha polypeptide a Protein, coagulation factor III Protein, platelet derived growth factor, alpha Protein, platelet derived growth factor subunit A S homeolog Protein, platelet-derived growth factor alpha polypeptide b Protein, PDGFA Protein, pdgfaa Protein, pdgfa Protein, F3 Protein, Pdgfa Protein, pdgfa.S Protein, pdgfab Protein
- 背景
- Alternative Names: PDGF-A, PDGF1, Platelet Derived Growth Factor Alpha Polypeptide
- 分子量
- 17kDa
- UniProt
- P20033
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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