MMP2 Protein (AA 110-660) (His tag)
-
- 抗原 See all MMP2 蛋白
- MMP2 (Matrix Metalloproteinase 2 (MMP2))
- 生物活性
- Active
- 产品特性
- AA 110-660
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This MMP2 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Mechanism: MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 72kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of MMP2 (500ng, 125ng, 63ng, 32ng, 16ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate-polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP2 protein as negative control, and blood sample as positive control. Result 1: Gelatin hydrolysis by recombinant human MMP2 was shown in figure 1. MMP2 MMP2 MMP2 MMP2 MMP2 negative positive 500ng 125ng 63ng 32ng 16ng control control 70kd→ Figure 1. Hydrolysis of gelatin by MMP2.
- Top Product
- Discover our top product MMP2 蛋白
-
Matrix Metalloproteinase 2 (MMP2) (Active) protein
MMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Matrix Metalloproteinase 2 (MMP2) (AA 34-660) protein (His tag)MMP2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metalloproteinase 2 (MMP2) (AA 424-660) protein (His-IF2DI Tag)MMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Matrix Metalloproteinase 2 (MMP2) (AA 1-660) protein (GST tag)MMP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Matrix Metalloproteinase 2 (MMP2) (AA 561-660) protein (GST tag)MMP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Matrix Metalloproteinase 2 (MMP2) (AA 109-660) protein (His tag)MMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Matrix Metalloproteinase 2 (MMP2) (AA 445-662) protein (His tag)Crystallography grade MMP2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Matrix Metalloproteinase 2 (MMP2) (AA 110-660) protein (His tag)Crystallography grade MMP2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Matrix Metalloproteinase 2 (MMP2) (AA 110-662) protein (His tag)Crystallography grade MMP2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- 应用备注
- Isoelectric Point: 5.2
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
- 抗原
- MMP2 (Matrix Metalloproteinase 2 (MMP2))
- Abstract
- MMP2 产品
- 别名
- 2-MMP Protein, CG1794 Protein, DM2-MMP Protein, Dm2-MMP Protein, Dmel\\CG1794 Protein, MMP2 Protein, anon-WO0118547.84 Protein, dm-2MMP Protein, dmmp2 Protein, l(2)02353 Protein, mmp2 Protein, wu:fa99h12 Protein, wu:fk89d01 Protein, fgmmp-2 Protein, Mmp-2 Protein, LOC100135793 Protein, Clg4a Protein, GelA Protein, MMP-2 Protein, CLG4 Protein, CLG4A Protein, MMP-II Protein, MONA Protein, TBE-1 Protein, matrix metallopeptidase 2 Protein, Matrix metalloproteinase 2 Protein, matrix metalloproteinase 2 Protein, matrix metalloproteinase-2 Protein, MMP2 Protein, Mmp2 Protein, mmp2 Protein, LOC657982 Protein, LOC100135793 Protein
- 背景
- Alternative Names: CLG4, CLG4A, MMP-II, MONA, TBE-1, PEX, Gelatinase A, 72 kDa Gelatinase, 72 kDa Type IV Collagenase
- 分子量
- 64kDa
- UniProt
- P08253
- 途径
- Activation of Innate immune Response
-
