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CSTF1 Protein (AA 1-431) (His tag)
CSTF1
宿主: 人
宿主: Baculovirus infected Insect Cells
Recombinant
> 90 % by SDS - PAGE
SDS
产品细节
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抗原
See all CSTF1 蛋白
CSTF1
(Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1))
蛋白类型
Recombinant
产品特性
AA 1-431
宿主
All origins for CSTF1 蛋白
人
资源
All sources for CSTF1 蛋白
Baculovirus infected Insect Cells
标记
This CSTF1 protein is labelled with His tag.
应用范围
SDS-PAGE (SDS)
序列
MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DHHHHHH
纯度
> 90 % by SDS - PAGE
内毒素水平
< 1.0 EU per 1 microgram of protein (determined by LAL method)
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Alternatives
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
贮存及处理
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状态
Liquid
浓度
0.5 mg/mL
缓冲液
Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 1 mM DTT, 20 % glycerol.
储存条件
4 °C,-20 °C,-80 °C
储存方法
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
目标详细情况
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抗原
CSTF1
(Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1))
别名
CSTF1 (CSTF1 产品 )
别名
fc22d10 Protein, zgc:66207 Protein, zgc:77560 Protein, wu:fc22d10 Protein, wu:fd16h09 Protein, MGC53063 Protein, MGC69421 Protein, MGC83790 Protein, 1700057K18Rik Protein, AI788832 Protein, CstF-50 Protein, CstFp50 Protein, cleavage stimulation factor, 3' pre-RNA, subunit 1 Protein, cleavage stimulation factor subunit 1 Protein, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa Protein, cstf1 Protein, cstf1.S Protein, CSTF1 Protein, cstf1.L Protein, Cstf1 Protein
背景
CSTF1, also known as cleavage stimulation factor subunit 1, is one of the multiple factors required for polyadenylation of mammalian pre-mRNAs in vitro. It is functioning with cleavage-polyadenylation specificity factor, two cleavage factors, and poly(A)+ polymerase. It is involved in the 3' end processing of messenger RNA precursors. Recombinant human CSTF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
分子量
49.1kDa (437aa) 40-57kDa (SDS-PAGE under reducing conditions)
NCBI登录号
NP_001028693
UniProt
Q05048
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