CST5 Protein (AA 26-142) (His tag)
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- 抗原 See all CST5 蛋白
- CST5 (Cystatin D (CST5))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 26-142
- 宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This CST5 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- QSRTLAGGIH ATDLNDKSVQ RALDFAISEY NKVINKDEYY SRPLQVMAAY QQIVGGVNYY FNVKFGRTTC TKSQPNLDNC PFNDQPKLKE EEFCSFQINE VPWEDKISIL NYKCRKVHHH HHH
- 纯度
- > 90 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- The IC50 value is < 2.0nM. The inhibitory function of Cystatin 5 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
- Top Product
- Discover our top product CST5 蛋白
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Cystatin D (CST5) (AA 1-142) protein (GST tag)
CST5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cystatin D (CST5) (AA 21-142) protein (His tag)Crystallography grade CST5 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In phosphate buffered saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- CST5 (Cystatin D (CST5))
- 别名
- CST5 (CST5 产品)
- 别名
- Cst10 Protein, Cystatin-5 Protein, cystatin D Protein, CST5 Protein, Cst5 Protein
- 背景
- CST5, also known as Cystatin-D, is a novel member of the cystatin superfamily. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Two alleles of the cystatin D gene (CST5), encoding protein variants with either Cys or Arg as residue 26 in their 122-residue polypeptide chains, are present in the population. Recombinant human CST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 14.36kDa (123aa) (13.5-18kDa in SDS-PAGE under reducing conditions.)
- NCBI登录号
- NP_001891
- UniProt
- P28325
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