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IL12 Protein (AA 23-219, AA 23-328) (His tag)

IL12 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS Active
产品编号 ABIN5853918
发货至: 中国
  • 抗原 See all IL12 蛋白
    IL12 (Interleukin 12 (IL12))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 23-219, AA 23-328
    宿主
    • 17
    • 9
    • 5
    • 1
    资源
    • 14
    • 5
    • 4
    • 4
    • 2
    Baculovirus infected Insect Cells
    标记
    This IL12 protein is labelled with His tag.
    应用范围
    SDS-PAGE (SDS)
    序列
    * IL-12 is a heterodime r of IL12A and IL12B linked through a disulfide-bond between cysteines in red in below sequences.
    IL12A(p35)
    RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH
    IL12B(p40)
    IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
    纯度
    > 90 % by SDS - PAGE
    内毒素水平
    < 1.0 EU per 1 microgram of protein (determined by LAL method)
    Biological Activity Comment
    The activity is determined by the IFN-g ELISA in a using NK-92 cell. The ED50 for this effect is less than or equal to 0.03 ng/ml.
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    Discover our top product IL12 蛋白
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    说明

    Bioactivity Validated

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol
    储存条件
    4 °C,-20 °C,-80 °C
    储存方法
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • 抗原
    IL12 (Interleukin 12 (IL12))
    别名
    IL12 (IL12 产品)
    别名
    CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, LOC100217488 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p40 Protein, IL12B Protein, IL-12 p35 Protein, interleukin 12B Protein, interleukin 12A Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12B Protein, IL12A Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein
    背景
    IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Recombinant human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.
    分子量
    34.6kDa (p40) & 23.3kDa (p35) (306aa & 203aa) The heterodimeric IL12 has a predicted molecular weight of 57.9kDa (509aa). Molecular weight on SDS-PAGE will appear higher.
    途径
    JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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