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IL-3 Protein (AA 20-152) (His tag)

IL-3 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS Active
产品编号 ABIN5778892
发货至: 中国
  • 抗原 See all IL-3 蛋白
    IL-3 (Interleukin 3 (IL-3))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 20-152
    宿主
    • 25
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    资源
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    Baculovirus infected Insect Cells
    标记
    This IL-3 protein is labelled with His tag.
    应用范围
    SDS-PAGE (SDS)
    序列
    APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIFLEHHHHH H
    纯度
    > 90 % by SDS - PAGE
    内毒素水平
    < 1.0 EU per 1 microgram of protein (determined by LAL method)
    Biological Activity Comment
    Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects less or equal to 1.5 ng/ml.
    Top Product
    Discover our top product IL-3 蛋白
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    说明

    Bioactivity Validated

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.
    储存条件
    4 °C,-20 °C,-80 °C
    储存方法
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • 抗原
    IL-3 (Interleukin 3 (IL-3))
    别名
    IL3 (IL-3 产品)
    别名
    BPA Protein, Csfmu Protein, HCGF Protein, Il-3 Protein, MCGF Protein, PSF Protein, IL-3 Protein, MULTI-CSF Protein, interleukin 3 Protein, interleukin 3 (colony-stimulating factor, multiple) Protein, Il3 Protein, IL3 Protein
    背景
    IL3, as known as interleukin 3, is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. Also, this protein affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulation factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factors and WEHI-3 growth factor. Recombinant human IL3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
    分子量
    16.1kDa (141aa) 13.5-18kDa (SDS-PAGE under reducing conditions)
    NCBI登录号
    NP_000579
    UniProt
    P08700
    途径
    JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
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