IL-3 Protein (AA 20-152) (His tag)
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- 抗原 See all IL-3 蛋白
- IL-3 (Interleukin 3 (IL-3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 20-152
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This IL-3 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIFLEHHHHH H
- 纯度
- > 90 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects less or equal to 1.5 ng/ml.
- Top Product
- Discover our top product IL-3 蛋白
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- IL-3 (Interleukin 3 (IL-3))
- 别名
- IL3 (IL-3 产品)
- 别名
- BPA Protein, Csfmu Protein, HCGF Protein, Il-3 Protein, MCGF Protein, PSF Protein, IL-3 Protein, MULTI-CSF Protein, interleukin 3 Protein, interleukin 3 (colony-stimulating factor, multiple) Protein, Il3 Protein, IL3 Protein
- 背景
- IL3, as known as interleukin 3, is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. Also, this protein affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulation factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factors and WEHI-3 growth factor. Recombinant human IL3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 16.1kDa (141aa) 13.5-18kDa (SDS-PAGE under reducing conditions)
- NCBI登录号
- NP_000579
- UniProt
- P08700
- 途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
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