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GDA Protein (AA 1-454) (His tag)

GDA 宿主: 小鼠 宿主: 大肠杆菌(E. Coli) Recombinant > 95 % by SDS - PAGE SDS Active
产品编号 ABIN5854994
发货至: 中国
  • 抗原 See all GDA 蛋白
    GDA (Guanine Deaminase (GDA))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 1-454
    宿主
    • 9
    • 3
    • 1
    • 1
    • 1
    • 1
    小鼠
    资源
    • 8
    • 4
    • 1
    • 1
    • 1
    • 1
    大肠杆菌(E. Coli)
    标记
    This GDA protein is labelled with His tag.
    应用范围
    SDS-PAGE (SDS)
    序列
    MGSSHHHHHH SSGLVPRGSH MGSMCAARTP PLALVFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE SSQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHAPQ YAFAGSNVDL PLLEWLNKYT FPTEQRFRST DVAEEVYTRV VRRTLKNGTT TACYFGTIHT DSSLILAEIT DKFGQRAFVG KVCMDLNDTV PEYKETTEES VKETERFVSE MLQKNYPRVK PIVTPRFTLS CTETLMSELG NIAKTHDLYI QSHISENREE IEAVKSLYPS YKNYTDVYDK NNLLTNKTVM AHGCYLSEEE LNIFSERGAS IAHCPNSNLS LSSGLLNVLE VLKHKVKIGL GTDVAGGYSY SMLDAIRRAV MVSNVLLINK VNEKNLTLKE VFRLATLGGS QALGLDSEIG NFEVGKEFDA LLINPRASDS PIDLFYGDFV GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV
    纯度
    > 95 % by SDS - PAGE
    Biological Activity Comment
    Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
    Top Product
    Discover our top product GDA 蛋白
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    说明

    Bioactivity Validated

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Liquid. In 20  mM Tris-HCl(  pH 8.0) containing 0.15M NaCl, 1  mM DTT, 10 % glycerol
    储存条件
    4 °C,-20 °C,-80 °C
    储存方法
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • 抗原
    GDA (Guanine Deaminase (GDA))
    别名
    Gda (GDA 产品)
    别名
    CYPIN Protein, GUANASE Protein, NEDASIN Protein, AU015411 Protein, AW047581 Protein, Cypin Protein, MGC81835 Protein, zgc:112282 Protein, AGR_C_4212 Protein, DDBDRAFT_0169373 Protein, DDBDRAFT_0230211 Protein, DDB_0169373 Protein, DDB_0230211 Protein, guanine deaminase Protein, guanine deaminase L homeolog Protein, short-chain dehydrogenase/reductase sdr Protein, GDA Protein, Gda Protein, gda.L Protein, gda Protein, SPO2956 Protein, Gdia_3192 Protein, Mchl_2046 Protein, Mnod_4655 Protein, guaD Protein, Dd586_1771 Protein, Kvar_2512 Protein, Mrub_1462 Protein, BC1002_2263 Protein, Cseg_1079 Protein, Mesil_1795 Protein, Trad_2850 Protein, Mesop_0346 Protein, gDA Protein, ML5_2802 Protein
    背景
    Gda, also known as guanine deaminase, catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant mouse Gda, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
    分子量
    53.4 kDa (477aa) Confirmed by MALDI-TOF
    NCBI登录号
    NP_034396
    UniProt
    Q9R111
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