Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Liquid
浓度
0.1-2 mg/mL
缓冲液
20 mM Tris-HCl based buffer, pH 8.0
储存条件
-80 °C,4 °C,-20 °C
储存方法
Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
抗原
MIB1
(Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))
E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis . Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.1 Publication