DDX58 Protein (AA 1-430, ATP-binding domain) (His tag)
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- 抗原 See all DDX58 蛋白
- DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-430, ATP-binding domain
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This DDX58 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MTTEQRRSLQ AFQDYIRKTL DPTYILSYMA PWFREEEVQY IQAEKNNKGP MEAATLFLKF LLELQEEGWF RGFLDALDHA GYSGLYEAIE SWDFKKIEKL EEYRLLLKRL QPEFKTRIIP TDIISDLSEC LINQECEEIL QICSTKGMMA GAEKLVECLL RSDKENWPKT LKLALEKERN KFSELWIVEK GIKDVETEDL EDKMETSDIQ IFYQEDPECQ NLSENSCPPS EVSDTNLYSP FKPRNYQLEL ALPAMKGKNT IICAPTGCGK TFVSLLICEH HLKKFPQGQK GKVVFFANQI PVYEQQKSVF SKYFERHGYR VTGISGATAE NVPVEQIVEN NDIIILTPQI LVNNLKKGTI PSLSIFTLMI FDECHNTSKQ HPYNMIMFNY LDQKLGGSSG PLPQVIGLTA SVGVGDAKNT DEALDYICKL
- 纯化方法
- SDS-PAGE
- 纯度
- > 90 %
- Top Product
- Discover our top product DDX58 蛋白
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DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 1-925) protein (GST tag)
DDX58 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 1-925) protein (Strep Tag)Crystallography grade DDX58 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 1-926) protein (Strep Tag)Crystallography grade DDX58 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) protein (Myc-DYKDDDDK Tag)DDX58 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) proteinDDX58 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1-2 mg/mL
- 缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
- 储存条件
- -80 °C,4 °C,-20 °C
- 储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- 抗原
- DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))
- 别名
- DDX58 (DDX58 产品)
- 别名
- RIG-I Protein, RIGI Protein, RLR-1 Protein, 6430573D20Rik Protein, C330021E21 Protein, RHIV-1 Protein, RIG-1 Protein, DExD/H-box helicase 58 Protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 Protein, DEXD/H-box helicase 58 Protein, DDX58 Protein, Ddx58 Protein
- 背景
- Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA (<,1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs), IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including mbers of the families Paramyxoviridae: Human respiratory syncytial virus and measles virus (MeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration
- 分子量
- 53.3 kDa
- UniProt
- O95786
- 途径
- Activation of Innate immune Response, Hepatitis C
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