AP2M1 Protein (AA 1-435, full length) (His-SUMO Tag)
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- 抗原 See all AP2M1 蛋白
- AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))
- 蛋白类型
- Recombinant
- 产品特性
- full length, AA 1-435
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This AP2M1 protein is labelled with His-SUMO Tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MIGGLFIYNH KGEVLISRVY RDDIGRNAVD AFRVNVIHAR QQVRSPVTNI ARTSFFHVKR SNIWLAAVTK QNVNAAMVFE FLYKMCDVMA AYFGKISEEN IKNNFVLIYE LLDEILDFGY PQNSETGALK TFITQQGIKS QHQTKEEQSQ ITSQVTGQIG WRREGIKYRR NELFLDVLES VNLLMSPQGQ VLSAHVSGRV VMKSYLSGMP ECKFGMNDKI VIEKQGKGTA DETSKSGKQS IAIDDCTFHQ CVRLSKFDSE RSISFIPPDG EFELMRYRTT KDIILPFRVI PLVREVGRTK LEVKVVIKSN FKPSLLAQKI EVRIPTPLNT SGVQVICMKG KAKYKASENA IVWKIKRMAG MKESQISAEI ELLPTNDKKK WARPPISMNF EVPFAPSGLK VRYLKVFEPK LNYSDHDVIK WVRYIGRSGI YETRC
- 纯化方法
- SDS-PAGE
- 纯度
- > 90 %
- Top Product
- Discover our top product AP2M1 蛋白
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Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 1-435) protein (GST tag)
AP2M1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 1-435) protein (Strep Tag)Crystallography grade AP2M1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 1-435) protein (Strep Tag)Crystallography grade AP2M1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 1-435), (full length) protein (GST tag)AP2M1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % SDS, ELISA, WB
Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 1-435), (full length) protein (His tag)AP2M1 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)AP2M1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1-2 mg/mL
- 缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
- 储存条件
- -80 °C,4 °C,-20 °C
- 储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- 抗原
- AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))
- 别名
- AP2M1 (AP2M1 产品)
- 别名
- AP50 Protein, CLAPM1 Protein, mu2 Protein, Ap50 Protein, Clapm1 Protein, cb34 Protein, Ap2m1 Protein, dpy-23 Protein, sb:cb34 Protein, zgc:55711 Protein, zgc:85653 Protein, wu:fa97a10 Protein, ap2m1-a Protein, ap2m1 Protein, zgc:56643 Protein, AP2M1 Protein, adaptor related protein complex 2 mu 1 subunit Protein, adaptor-related protein complex 2, mu 1 subunit Protein, adaptor-related protein complex 2, mu 1 subunit, a Protein, adaptor related protein complex 2 mu 1 subunit L homeolog Protein, adaptor-related protein complex 2, mu 1 subunit, b Protein, AP-2 complex subunit mu-1 Protein, clathrin coat assembly protein AP50 Protein, clathrin coat assembly protein ap50 Protein, AP2M1 Protein, Ap2m1 Protein, ap2m1a Protein, ap2m1.L Protein, ap2m1b Protein, ap2m1 Protein, cgd8_950 Protein, PY06523 Protein, PVX_118455 Protein, PVX_123590 Protein, CpipJ_CPIJ003697 Protein
- 背景
- Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different mbrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to mbrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of mbranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the mbrane proteins involved in receptor-mediated endocytosis. AP-2 ses to play a role in the recycling of synaptic vesicle mbranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmbrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmbrane cargo proteins, it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in mbrane-associated AP-2. The mbrane-specific phosphorylation event appears to involve assbled clathrin which activates the AP-2 mu kinase AAK1 . Plays a role in endocytosis of frizzled family mbers upon Wnt signaling .
- 分子量
- 65.6 kDa
- UniProt
- Q96CW1
- 途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome
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