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IL-21 Protein (AA 30-162) (Fc Tag,AVI tag,Biotin)

IL21 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
产品编号 ABIN5674598
发货至: 中国
  • 抗原 See all IL-21 (IL21) 蛋白
    IL-21 (IL21) (Interleukin 21 (IL21))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 30-162
    宿主
    • 31
    • 9
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    资源
    • 24
    • 15
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    标记
    This IL-21 protein is labelled with Fc Tag,AVI tag,Biotin.
    品牌
    PrecisionAvi
    序列
    AA 30-162
    特异性
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    产品特性
    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 43.9 kDa. The protein migrates as 36 kDa and 48-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    纯度
    >95 % as determined by SDS-PAGE.
    内毒素水平
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product IL21 蛋白
  • 说明

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5
    注意事项
    Please avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
  • 抗原
    IL-21 (IL21) (Interleukin 21 (IL21))
    别名
    IL-21 (IL21 产品)
    别名
    IL-21 Protein, Za11 Protein, interleukin 21 Protein, IL21 Protein, Il21 Protein
    背景
    Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
    分子量
    43.6 kDa
    NCBI登录号
    NP_001193935
    途径
    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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