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Interleukin 35 Protein (IL35) (AA 21-229, AA 23-219) (His tag)

IL35 宿主: 人 宿主: 大肠杆菌(E. Coli) > 97 % AcA, CC Active
产品编号 ABIN5665857
发货至: 中国
  • 抗原 See all Interleukin 35 (IL35) 蛋白
    Interleukin 35 (IL35)
    生物活性
    Active
    产品特性
    AA 21-229, AA 23-219
    宿主
    • 6
    • 2
    资源
    • 5
    • 2
    • 1
    • 1
    大肠杆菌(E. Coli)
    标记
    This Interleukin 35 protein is labelled with His tag.
    应用范围
    Activity Assay (AcA), Cell Culture (CC)
    产品特性
    Tag location: N-terminal His Tag
    纯度
    > 97 %
    Biological Activity Comment
    IL35 (Interleukin 35) is an IL-12 family cytokine, which is a dimeric protein composed of IL-12α and IL-27β chains. IL35 is thought to mediate the immune inhibitory function of regulatory T cells and has been proven to promotes pancreas cancer growth through enhancement of proliferation and inhibition of apoptosis. Thus, proliferation assay of IL35 was conducted using PANC-1 cells. Briefly, PANC-1 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of recombinant human IL35. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of PANC-1 cells after incubation with IIL35 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL35 for 48h. The result was shown in Figure 2. It was obvious that IL35 significantly increased cell viability of PANC-1 cells.
  • 应用备注
    Isoelectric Point: 8.7
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    储存液
    Dithiothreitol (DTT), Other preservative, ProClin
    注意事项
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • 抗原
    Interleukin 35 (IL35)
    Abstract
    IL35 产品
    分子量
    55kDa
    UniProt
    P29459
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