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- 抗原 See all IL-24 (IL24) 蛋白
- IL-24 (IL24) (Interleukin 24 (IL24))
- 生物活性
- Active
- 产品特性
- AA 51-206
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IL-24 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can induce apoptosis selectively in various cancer cells, including ECV304. Thus, inhibition of cell proliferation assay of IL24 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL24. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Inhibition of ECV-304 cells proliferation after incubation with IIL24 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of IL24 for 48h. The mean OD value of ECV-304 assessed by CCK-8 was shown in Figure 2. It was obvious that IL24 significantly decreased cell viability of ECV-304 cells.
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Interleukin 24 (IL24) (AA 51-206) (Active) protein (His tag)
IL24 宿主: 人 宿主: 大肠杆菌（E. Coli） > 90 % AcA, CC Active
Interleukin 24 (IL24) (AA 1-207) protein (GST tag)IL24 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 24 (IL24) (AA 1-206) protein (GST tag)IL24 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 24 (IL24) (AA 58-167) protein (GST tag)IL24 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 24 (IL24) (AA 52-206) protein (His tag)Crystallography grade IL24 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Interleukin 24 (IL24) (AA 27-181) protein (His tag)Crystallography grade IL24 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Isoelectric Point: 8.6
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- IL-24 (IL24) (Interleukin 24 (IL24))
- Abstract
- IL24 产品
- 别名
- C49A Protein, FISP Protein, IL10B Protein, MDA7 Protein, MOB5 Protein, ST16 Protein, Mda7 Protein, St16 Protein, Mda-7 Protein, IL24 Protein, interleukin 24 Protein, IL24 Protein, Il24 Protein
- 背景
- Alternative Names: C49A, FISP, IL10B, MDA7, Mob-5, ST16, Mda-7, Melanoma Differentiation Association Protein 7, Suppression Of Tumorigenicity 16
- 分子量
- 18kDa
- UniProt
- Q13007
- 途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Autophagy
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