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- 抗原 See all IL-20 (IL20) 蛋白
- IL-20 (IL20) (Interleukin 20 (IL20))
- 生物活性
- Active
- 产品特性
- AA 25-176
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IL-20 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- IL20 (Interleukin-20) is a cytokine structurally related to interleukin 10, which is produced by activated keratinocytes and monocytes. It is accepted that IL20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Thus, proliferation assay of IL20 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL20. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of ECV-304 cells after incubation with IIL20 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL20 for 48h. The result was shown in Figure 2. It was obvious that human IL20 significantly decreased cell viability of ECV-304 cells.
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- Discover our top product IL20 蛋白
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Interleukin 20 (IL20) (AA 25-176) protein (His tag)
IL20 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 20 (IL20) (AA 25-176) protein (Fc Tag)IL20 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 20 (IL20) (AA 25-176) protein (His tag)IL20 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 20 (IL20) (AA 25-176) protein (Fc Tag)IL20 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 20 (IL20) (AA 25-176) (Active) protein (His tag)IL20 宿主: 人 宿主: 大肠杆菌（E. Coli） > 90 % AcA, CC Active
Interleukin 20 (IL20) (AA 1-176) protein (GST tag)IL20 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 20 (IL20) (AA 67-176) protein (GST tag)IL20 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 20 (IL20) (AA 25-176) protein (His tag)Crystallography grade IL20 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Interleukin 20 (IL20) (AA 25-176) protein (His tag)Crystallography grade IL20 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Isoelectric Point: 8.9
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- IL-20 (IL20) (Interleukin 20 (IL20))
- Abstract
- IL20 产品
- 别名
- IL-20 Protein, IL10D Protein, ZCYTO10 Protein, Zcyto10 Protein, IL20 Protein, interleukin 20 Protein, IL20 Protein, Il20 Protein
- 背景
- Alternative Names: IL10D, IL10-D, ZCYTO10, Cytokine Zcyto10
- 分子量
- 21kDa
- UniProt
- Q9NYY1
- 途径
- Protein targeting to Nucleus
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