IL-15 Protein (AA 50-162) (His tag)
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- 抗原 See all IL-15 (IL15) 蛋白
- IL-15 (IL15) (Interleukin 15 (IL15))
- 生物活性
- Active
- 产品特性
- AA 50-162
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IL-15 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Interleukin 15 (IL15) is a widely expressed cytokine that is structurally and functionally related to IL2, which plays an important role in many immunological diseases. IL15 also regulates T and natural killer (NK) cell activation and proliferation. To test the effect of IL15 on cells proliferation of human T lymphocyte cells, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL15. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL15 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of IL15 was shown in Figure 2. It was obvious that IL15 significantly promoted cell proliferation of Jurkat cells. The ED50 for this effect is typically 0.7240 to 5.206ng/mL.
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Interleukin 15 (IL15) protein
IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by SEC-HPLC
Interleukin 15 (IL15) (AA 50-162) (Active) protein (His tag)IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Interleukin 15 (IL15) (AA 49-162) protein (His-IF2DI Tag)IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 15 (IL15) (AA 49-162) protein (His-IF2DI Tag)IL15 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 15 (IL15) (AA 1-162) protein (GST tag)IL15 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 15 (IL15) (AA 30-129) protein (GST tag)IL15 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 15 (IL15) (AA 49-162) (Active) protein (His tag)IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Interleukin 15 (IL15) proteinIL15 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag)IL15 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (His tag)IL15 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- 应用备注
- Isoelectric Point: 5.1
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- IL-15 (IL15) (Interleukin 15 (IL15))
- Abstract
- IL15 产品
- 别名
- IL15 Protein, AI503618 Protein, IL-15 Protein, interleukin 15 Protein, IL15 Protein, Il15 Protein
- 分子量
- 14/16/18kDa
- UniProt
- P40933
- 途径
- JAK/STAT Signaling, Glycosaminoglycan Metabolic Process
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