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FGF1 Protein (AA 1-155) (His tag,GST tag)

FGF1 宿主: 人 宿主: 大肠杆菌(E. Coli) > 97 % AcA, CC Active
产品编号 ABIN5665794
发货至: 中国
  • 抗原 See all FGF1 蛋白
    FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
    生物活性
    Active
    产品特性
    AA 1-155
    宿主
    • 21
    • 5
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    资源
    • 28
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    大肠杆菌(E. Coli)
    标记
    This FGF1 protein is labelled with His tag,GST tag.
    应用范围
    Activity Assay (AcA), Cell Culture (CC)
    产品特性
    Tag location: N-terminal His and GST Tag
    纯度
    > 97 %
    Biological Activity Comment
    FGF1 (Fibroblast growth factor 1) is a member of FGF family, which plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts, besides, human FGF1 and mouse FGF1 shares similarities in amino acid sequence with the identity of 96%. Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant human FGF1 using 3T3 fibroblasts. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. The result was shown in Figure 2. It was obvious that FGF1 significantly increased cell viability of 3T3 cells.
    Top Product
    Discover our top product FGF1 蛋白
  • 应用备注
    Isoelectric Point: 6.5
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    储存液
    Dithiothreitol (DTT), Other preservative, ProClin
    注意事项
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • 抗原
    FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
    别名
    Fibroblast Growth Factor 1, Acidic (FGF1) (FGF1 产品)
    别名
    AFGF Protein, ECGF Protein, ECGF-beta Protein, ECGFA Protein, ECGFB Protein, FGF-1 Protein, FGF-alpha Protein, FGFA Protein, GLIO703 Protein, HBGF-1 Protein, HBGF1 Protein, Fgf1 Protein, FGF1 Protein, aFGF Protein, ecgf Protein, fgfa Protein, ecgfa Protein, ecgfb Protein, fgf-1 Protein, hbgf1 Protein, glio703 Protein, ecgf-beta Protein, fgf-alpha Protein, Dffrx Protein, Fam Protein, Fgf-1 Protein, Fgfa Protein, zgc:136885 Protein, LOC100221393 Protein, fgf1 Protein, zgc:73249 Protein, fibroblast growth factor 1 Protein, fibroblast growth factor 1 L homeolog Protein, fibroblast growth factor 1b Protein, fibroblast growth factor 1a Protein, FGF1 Protein, fgf1.L Protein, fgf1 Protein, Fgf1 Protein, fgf1b Protein, LOC100221393 Protein, fgf1a Protein
    背景
    Alternative Names: ECGF, AFGF, ECGFA, ECGFB, FGF-Alpha, FGFA, HBGF1, ECGFB, HBGF1, Acidic Fibroblast Growth Factor, heparin-binding growth factor 1, Endothelial Cell Growth Factor, Beta
    分子量
    55kDa
    UniProt
    P05230
    途径
    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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