ERK1 Protein (pThr202, pTyr204)
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- 抗原 See all ERK1 (MAPK3) 蛋白
- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
- 蛋白类型
- Recombinant
- 产品特性
- pThr202, pTyr204
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宿主
- 人, 大鼠, 小鼠
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资源
- Insect cells (Sf9)
- 应用范围
- Western Blotting (WB)
- 纯化方法
- ERK1 pT202-pY204 is a recombinant protein containing a polyhistidine tag expressed in Sf9. ERK1 was phosphorylated in vitro using the activated MEK. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK1 and was estimated to be greater than 95% pure.
- 过滤
- Sterile filtered
- Top Product
- Discover our top product MAPK3 蛋白
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- 应用备注
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Application Note: ERK1 pT202-pY204 is an activated recombinant protein suitable as a control for polyclonal or monoclonal anti-ERK in immunological assays. ERK1 can be activated by in vitro phosphorylation by MEK proteins. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Stabilizer: None
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
- 别名
- ERK1 (MAPK3 产品)
- UniProt
- P27361
- 途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
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