AKT1 Protein (Ser473Ala-Mutant, Thr308Ala-Mutant)
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- 抗原 See all AKT1 蛋白
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- 蛋白类型
- Recombinant
- 产品特性
- Ser473Ala-Mutant, Thr308Ala-Mutant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 应用范围
- Western Blotting (WB)
- 纯化方法
- Recombinant protein mutant corresponds to amino acids 1 to 480 of mature human AKT1, Akt isoform 1. There are two point mutation of key activating residues, T308A and S473A. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 106-480) (Active) protein (His-GST)
AKT1 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) protein (His tag)AKT1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >80 % as determined by reducing SDS-PAGE.
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-149) protein (GST tag)AKT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) protein (GST tag)AKT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 381-480) protein (GST tag)AKT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 127-200) protein (GST tag)AKT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) protein (His-IF2DI Tag)AKT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) protein (Strep Tag)Crystallography grade AKT1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)AKT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Application Note: AKT1 mutant protein is suitable as a control protein for immunoassays using antibodies targeting the T308 or S473 key phosphorylation sites. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.1 mg/mL
- 缓冲液
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Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol
Stabilizer: 10 % (v/v) Glycerol
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
- Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 12 months
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- 抗原
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- 别名
- AKT1 Protein (AKT1 产品)
- 别名
- AKT Protein, CWS6 Protein, PKB Protein, PKB-ALPHA Protein, PRKBA Protein, RAC Protein, RAC-ALPHA Protein, Akt Protein, Rac Protein, PKB/Akt Protein, PKBalpha Protein, AKT/PKB Protein, AKT1 Protein, Akt/PKB Protein, Akt1 Protein, CG4006 Protein, D-Akt Protein, DAKT1 Protein, DAKT1/PKB Protein, DAkt Protein, DAkt1 Protein, DPKB Protein, DRAC-PK Protein, DRAC-PK66 Protein, DRAC-PK85 Protein, Dakt Protein, Dakt1 Protein, Dmel\\CG4006 Protein, Dpkb Protein, PKB/AKT Protein, PKB/dAKT Protein, RacPK Protein, akt Protein, akt1 Protein, dAKT Protein, dAKT/dPKB Protein, dAKT1 Protein, dAkt Protein, dAkt/PKB Protein, dAkt1 Protein, dPKB Protein, dakt Protein, dakt1 Protein, l(3)04226 Protein, l(3)89Bq Protein, p-Akt Protein, pAkt Protein, ACT-5 Protein, akt-1 Protein, pkb Protein, v-akt Protein, v-akt1 Protein, xAct Protein, ATAKT1 Protein, F18A8.2 Protein, F18A8_2 Protein, K+ transporter 1 Protein, POTASSIUM TRANSPORTER Protein, AKT serine/threonine kinase 1 Protein, thymoma viral proto-oncogene 1 Protein, CG4006 gene product from transcript CG4006-RE Protein, actin beta Protein, v-akt murine thymoma viral oncogene homolog 1 S homeolog Protein, v-akt murine thymoma viral oncogene homolog 1 Protein, K+ transporter 1 Protein, Serine/threonine-protein kinase akt-1 Protein, AKT1 Protein, Akt1 Protein, ACTB Protein, akt1.S Protein, akt1 Protein, KT1 Protein, akt-1 Protein
- 基因ID
- 207
- UniProt
- P31749
- 途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
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