IDO1 Protein (AA 1-403) (His tag)
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- 抗原 See all IDO1 蛋白
- IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-403
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IDO1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 交叉反应
- 人
- 产品特性
- Human IDO (aa 1-403) is fused at the N-terminus to a His-tag.
- 纯度
- >90 % (SDS-PAGE)
- 过滤
- 0.2 μm filtered
- 内毒素水平
- <0.1EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product IDO1 蛋白
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Indoleamine 2,3-Dioxygenase 1 (IDO1) (AA 1-403) protein (His tag)
IDO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Indoleamine 2,3-Dioxygenase 1 (IDO1) (AA 1-403) protein (GST tag)IDO1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Indoleamine 2,3-Dioxygenase 1 (IDO1) protein (His tag)IDO1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Indoleamine 2,3-Dioxygenase 1 (IDO1) (AA 304-403) protein (GST tag)IDO1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Indoleamine 2,3-Dioxygenase 1 (IDO1) (Active) proteinIDO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % as determined by reducing SDS-PAGE. Active
Indoleamine 2,3-Dioxygenase 1 (IDO1) (AA 1-403) protein (Strep Tag)Crystallography grade IDO1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Indoleamine 2,3-Dioxygenase 1 (IDO1) (AA 1-407) protein (Strep Tag)Crystallography grade IDO1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Indoleamine 2,3-Dioxygenase 1 (IDO1) (Active) protein (Myc-DYKDDDDK Tag)IDO1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Specific Activity: >100'000U/mg protein with L-tryptophan as substrate (activity assay with catalase). One unit is defined as the amount of enzyme that produces 1nmol of N-formylkynurenine (NFK) per hour. For more information see Activity Test Protocol.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.2μm-filtered solution in 10 mM Tris, pH 7.5, containing 100 mM NaCl and 20 % glycerol.
- 储存条件
- -20 °C,-80 °C
- 储存方法
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Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 1 year after receipt when stored at -80°C. - 有效期
- 12 months
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- 抗原
- IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))
- 别名
- IDO (IDO1 产品)
- 背景
- IDO is a heme enzyme that catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway, causing depletion of tryptophan which can lead to halted growth of microbes as well as T cells. IDO is an immune checkpoint protein, thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation and antioxidant activity. Cancer cells are able to evade the immune system is by hijacking the checkpoint proteins. Increased IDO protein levels drive growth arrest and apoptosis of the effector T cells, a group of immune cells that mediate the immune system's ability to destroy pathogens. By reducing the number of effector T cells, IDO overexpression prevents the immune system from effectively destroying cancer cells. IDO overexpression has been observed in a wide range of human cancers such as prostatic, colorectal, pancreatic, cervical, gastric, ovarian, head or lung cancer. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders. Biological active IDO enzyme can be used for screening of IDO inhibitors, which have shown promising antitumor activity and appear to be synergistic in combination with chemotherapy and other forms of immunotherapy.
- 分子量
- ~47kDa (SDS-PAGE)
- UniProt
- P14902
- 途径
- Activated T Cell Proliferation
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