WNT3A Protein (AA 19-352)
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- 抗原 See all WNT3A 蛋白
- WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 19-352
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宿主
- 小鼠
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资源
- Mammalian Cells
- 应用范围
- Functional Studies (Func)
- 特异性
- Stem cell or organoid culture
- 纯化方法
- This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.
- 纯度
- 70-75 %
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg)
- Biological Activity Comment
- Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a (Lot: 02DEC2015) generate 100- fold increase of luciferase activity compared to control (buffer without Wnt3a). EC50 is about 6 ng/mL.
- Top Product
- Discover our top product WNT3A 蛋白
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Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 1-385) protein (GST tag)
WNT3A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 19-352) protein (His-IF2DI Tag)WNT3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 19-352) protein (His tag)Crystallography grade WNT3A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 19-351), (partial) protein (His tag)WNT3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 应用备注
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30-60 ng/mL
To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA
- 注意事项
- Mix the protein by pipetting up and down but not by vortexing
- 储存条件
- -20 °C
- 储存方法
- Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.
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- 抗原
- WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
- 别名
- Wnt3a (WNT3A 产品)
- 别名
- WNT3A Protein, Wnt-3a Protein, vt Protein, Zwnt[a] Protein, wnt3 Protein, wnt3 l Protein, wnt3l Protein, wnt[a] Protein, Xwnt-3a Protein, wnt-3a Protein, wnt3a-A Protein, xwnt3a Protein, WNT-3A Protein, WNT3 Protein, wingless-type MMTV integration site family, member 3A Protein, Wnt family member 3A Protein, wingless-type MMTV integration site family member 3A L homeolog Protein, WNT3A Protein, Wnt3a Protein, wnt3a Protein, wnt3a.L Protein
- 背景
- Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene
- 分子量
- 41 kDa evaluated by SDS-PAGE under reducing conditions
- 基因ID
- 22416
- NCBI登录号
- NC_000077
- UniProt
- P27467
- 途径
- WNT signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
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