Phospholipase B Protein (PLB) (AA 24-1478) (rho-1D4 tag)
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- 抗原 See all Phospholipase B (PLB) 蛋白
- Phospholipase B (PLB)
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-1478
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This Phospholipase B protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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QIHGSSGENT LAWQSQQVFW TLKNFPFPCK PKKLELSVLS ESVHSLRPSD IKLVAAIGNP EIPLAPGSGT INMEKPQSIK NQPQDVCMGI MTVLSDIIRH FSPSVLMPTC SPGKGTAVHT TAEDLWIQAK ELVRRLKDNP QLDFEKDWKL ITVFFSNTSQ CHLCPSAQQK SHLMRHMEML WGVLDYLHHE VPRAFVNLVD LSEVLAMDLQ HQETGFSPAP EVCKCTETTT LSKAVMQWSY QEAWEDLLAS SKFNKHETFA VVFQPFFDEI EPPLKRSSPQ DPTTLALRIW NSMMEPVGQK DGLLNTAERK TMKCPSEESP YLFTYKNSNY QARRLKPITK LQMKEGSEFT CPDKNPSNSI PTTVHSLRPA DIKIIGALGD SLTAGNGAGA SPWNILDVLT EYRGLSWSVG GDETIKTVTT LPNILREFNP SLKGFSVGTG KESTSRASFN QAVAGAKSDG LAGQARKLVD LMKADKTINF QEDWKIITVF IGGNDLCASC SNSTRFSPQN FIDNIKNALD ILHAEVPRAF VNMAMVMEIT PLRELFNEPT VSCPRNILSR LCPCVLGLGD NSEELSSLVQ RNRDYQKKTE ELINSGRYDT RDNFTVVVQP LFENVSMPRT PEGVPDKSFF APDCFHFNAK THARSAIALW KNMLEPVGHK TRHNNFEIKA PIVCPNQASP FLSTTKNSNL GNGTWMVCEE RAPSASPPTS VHTLRPADIQ VVAALGDSLT AGNGISSQEG NLTDVSTQYR GLSYSAGGDK TLENVTTLPN ILRKFNGNLT GYSVGTGDSS SANAFLNQAV PGAKAENLTS QVRTLVQKMK SDNRVNFNRD WKVITVMIGA SDLCDFCTDS NHYSAANFFD HLQNALDILH KEVPRALVNL VDFINPSIIR EVFLKNPDKC PVNQSSVLCN CVLTPRKDSY ELARLEAFTK SYQSSMLQLV ESGRYDTRED FSVVLQPFLL NTKLPVLENG KPDTSFFAPD CIHLNQKFHT QLARALWANM LEPLGKKTDT LDPKGHISLA CPTKDQPFLR TFRNSNYKYP TKPAIENWGS DFLCTEKSPS SQVPTSVHEL RPADIKVVAA MGDFLTTATG ARPSGYKRLA TPWRGLSWSI GGDGKLETHT TLPNILKKFN PSITGFSTGT LDNKAGLNVA EEGARAQDMP AQAKTLVKKM KSTPTINLQE DWKLITLLIG NNDLCLYCEN PEDNSTKEYV KYIQQALDIL YEELPRVFIN VVEVMELAGL HHVQGGKCAM PLAVQKNCSC LRHSQNLTAM QELKKLNWNL QSGISELSYW HRYMEREDFA VTVQPFFRNT FIPLNEREGL DLTFFSEDCF YFSDRGHAEM AIALWNNMLE PVGWKTSSNN FIYNRTKLKC PSPERPFLYT LRNSQLLPDK AEEPSNALYW AVPVAAIGGL AVGILGVMLW RTVKPVQQEE EEEDTLPNTS VTQDAVSEKR LKAGN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Plb1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product PLB 蛋白
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Phospholipase B (PLB) (AA 1-423) protein (GST tag)
PLB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Phospholipase B (PLB) (AA 1-488) protein (GST tag)PLB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Phospholipase B (PLB) (AA 22-1417) protein (His tag)Crystallography grade PLB 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Phospholipase B (PLB) (AA 24-1422) protein (His tag)Crystallography grade PLB 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Phospholipase B (PLB) (AA 22-1458) protein (rho-1D4 tag)Crystallography grade PLB 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Phospholipase B (PLB) protein (Myc-DYKDDDDK Tag)PLB 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Phospholipase B (PLB)
- 别名
- Plb1 (PLB 产品)
- 别名
- CMD1P Protein, CMH18 Protein, PLB Protein, PLB/LIP Protein, hPLB Protein, Phlpb Protein, 4632413E21Rik Protein, 4930433E17Rik Protein, 4930539A06Rik Protein, BC033606 Protein, PLB1 Protein, PHLPB Protein, phospholamban Protein, phospholipase B1 Protein, phospholipase B Protein, PLN Protein, PLB1 Protein, Plb1 Protein
- 背景
- Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity). {ECO:0000250}.
- 分子量
- 163.4 kDa Including tag.
- UniProt
- Q3TTY0
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