DMPK Protein (AA 1-631) (Strep Tag)
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- 抗原 See all DMPK 蛋白
- DMPK (Dystrophia Myotonica-Protein Kinase (DMPK))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-631
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This DMPK protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
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MSAEVRLRQL QQLVLDPGFL GLEPLLDLLL GVHQELGASH LAQDKYVADF LQWVEPIAAR LKEVRLQRDD FEILKVIGRG AFSEVAVVKM KQTGQVYAMK IMNKWDMLKR GEVSCFREER DVLVKGDRRW ITQLHFAFQD ENYLYLVMEY YVGGDLLTLL SKFGERIPAE MARFYLAEIV MAIDSVHRLG YVHRDIKPDN ILLDRCGHIR LADFGSCLKL QPDGMVRSLV AVGTPDYLSP EILQAVGGGP GAGSYGPECD WWALGVFAYE MFYGQTPFYA DSTAETYAKI VHYREHLSLP LADTVVPEEA QDLIRGLLCP AEIRLGRGGA GDFQKHPFFF GLDWEGLRDS VPPFTPDFEG ATDTCNFDVV EDRLTAMVSG GGETLSDMQE DMPLGVRLPF VGYSYCCMAF RDNQVPDPTP MELEALQLPV SDLQGLDLQP PVSPPDQVAE EADLVAVPAP VAEAETTVTL QQLQEALEEE VLTRQSLSRE LEAIRTANQN FSSQLQEAEV RNRDLEAHVR QLQERMEMLQ APGAAAITGV PSPRATDPPS HLDGPPAVAV GQCPLVGPGP MHRRHLLLPA RIPRPGLSEA RCLLLFAAAL AAAATLGCTG LVAYTGGLTP VWCFPGATFA P
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product DMPK 蛋白
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Dystrophia Myotonica-Protein Kinase (DMPK) (AA 1-544) (Active) protein (GST tag)
DMPK 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Dystrophia Myotonica-Protein Kinase (DMPK) (AA 1-629) protein (GST tag)DMPK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Dystrophia Myotonica-Protein Kinase (DMPK) (AA 1-629) protein (Strep Tag)Crystallography grade DMPK 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Dystrophia Myotonica-Protein Kinase (DMPK) (AA 1-590) protein (His tag)Crystallography grade DMPK 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Dystrophia Myotonica-Protein Kinase (DMPK) (AA 1-592) protein (His tag)Crystallography grade DMPK 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Dystrophia Myotonica-Protein Kinase (DMPK) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DMPK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- DMPK (Dystrophia Myotonica-Protein Kinase (DMPK))
- 别名
- Dmpk (DMPK 产品)
- 别名
- DMPK Protein, DM Protein, DM1 Protein, DM1PK Protein, DMK Protein, MDPK Protein, MT-PK Protein, Dm15 Protein, DM1 protein kinase Protein, dystrophia myotonica-protein kinase Protein, DMPK Protein, Dmpk Protein
- 背景
- Myotonin-protein kinase (MT-PK) (EC 2.7.11.1) (DM-kinase) (DMK) (DMPK) (Myotonic dystrophy protein kinase) (MDPK),FUNCTION: Non-receptor serine/threonine protein kinase which is necessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity. {ECO:0000269|PubMed:12612014, ECO:0000269|PubMed:15598648, ECO:0000269|PubMed:18729234, ECO:0000269|PubMed:21949239, ECO:0000269|PubMed:9294109}.
- 分子量
- 69.6 kDa
- UniProt
- P54265
- 途径
- Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development
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