KCNJ5 Protein (AA 1-419) (Strep Tag)
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- 抗原 See all KCNJ5 蛋白
- KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-419
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This KCNJ5 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), ELISA, SDS-PAGE (SDS)
- 序列
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MAGDSRNAMN QDMEIGVTSQ DHKKIPKQAR DYIPIATDRT RLLTEGKKPR QRYMEKTGKC NVHHGNVQET YRYLSDLFTT LVDLKWRFNL LVFTMVYTIT WLFFGFIWWL IAYVRGDLDH VGDQEWIPCV ENLSGFVSAF LFSIETETTI GYGFRVITEK CPEGIILLLV QAILGSIVNA FMVGCMFVKI SQPKKRAETL MFSNNAVISM RDEKLCLMFR VGDLRNSHIV EASIRAKLIK SRQTKEGEFI PLNQTDINVG FDTGDDRLFL VSPLIISHEI NEKSPFWEMS RAQLEQEEFE VVVILEGMVE ATGMTCQARS SYMDTEVLWG HRFTPVLTLE KGFYEVDYNT FHDTYETNTP SCCAKELAEM KRSGRLLQYL PSPPLLGGCA EAGNEAEAEK DEEGEPNGLS VSQATRGSM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product KCNJ5 蛋白
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Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5) (AA 321-419) protein (GST tag)
KCNJ5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5) (AA 136-204) protein (GST tag)KCNJ5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5) (AA 1-450) protein (GST tag)KCNJ5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5) (AA 1-419) protein (Strep Tag)Crystallography grade KCNJ5 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5) (AA 186-419) protein (His tag)Crystallography grade KCNJ5 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5) (AA 186-419) protein (His tag)Crystallography grade KCNJ5 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5) protein (Myc-DYKDDDDK Tag)KCNJ5 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))
- 别名
- Kcnj5 (KCNJ5 产品)
- 别名
- CIR Protein, GIRK4 Protein, KATP1 Protein, KIR3.4 Protein, LQT13 Protein, Kir3.4 Protein, GIRK-4 Protein, KATP-1 Protein, potassium voltage-gated channel subfamily J member 5 Protein, potassium inwardly-rectifying channel, subfamily J, member 5 Protein, KCNJ5 Protein, Kcnj5 Protein
- 背景
- G protein-activated inward rectifier potassium channel 4 (GIRK-4) (Cardiac inward rectifier) (CIR) (Heart KATP channel) (Inward rectifier K(+) channel Kir3.4) (KATP-1) (Potassium channel, inwardly rectifying subfamily J member 5),FUNCTION: This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium, as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium. {ECO:0000269|PubMed:7499385}.
- 分子量
- 47.7 kDa
- UniProt
- P48545
- 途径
- Notch Signaling
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