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SOCS1 Protein (AA 1-212) (His tag)

Crystallography grade SOCS1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
产品编号 ABIN3147837
发货至: 中国
  • 抗原 See all SOCS1 蛋白
    SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))
    蛋白类型
    Recombinant
    产品特性
    AA 1-212
    宿主
    • 6
    • 2
    • 1
    小鼠
    资源
    • 5
    • 2
    • 1
    • 1
    Insect Cells
    标记
    This SOCS1 protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    序列
    MVARNQVAAD NAISPAAEPR RRSEPSSSSS SSSPAAPVRP RPCPAVPAPA PGDTHFRTFR SHSDYRRITR TSALLDACGF YWGPLSVHGA HERLRAEPVG TFLVRDSRQR NCFFALSVKM ASGPTSIRVH FQAGRFHLDG SRETFDCLFE LLEHYVAAPR RMLGAPLRQR RVRPLQELCR QRIVAAVGRE NLARIPLNPV LRDYLSSFPF QIHHHHHH
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Socs1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    The protein is purified from the cleared cell lysate using His-tag capture materials.Eluate fractions are analyzed by SDS-PAGE.Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    20 mM Hepes, pH 7.4, 100 mM NaCl
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))
    别名
    Socs1 (SOCS1 产品)
    别名
    socs1 Protein, zgc:91868 Protein, jab Protein, cis1 Protein, ssi1 Protein, tip3 Protein, cish1 Protein, ssi-1 Protein, socs-1 Protein, SOCS1 Protein, LOC100228306 Protein, socs1b Protein, CIS1 Protein, CISH1 Protein, JAB Protein, SOCS-1 Protein, SSI-1 Protein, SSI1 Protein, TIP3 Protein, Cish1 Protein, Cish7 Protein, Socs-1 Protein, suppressor of cytokine signaling 1a Protein, suppressor of cytokine signaling 1 Protein, suppressor of cytokine signaling 1 L homeolog Protein, socs1a Protein, socs1 Protein, SOCS1 Protein, socs1.L Protein, Socs1 Protein
    背景
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity (By similarity). Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2 (By similarity). SOCS1 appears to be a negative regulator in IGF1R signaling pathway (By similarity). {ECO:0000250, ECO:0000269|PubMed:10064597, ECO:0000269|PubMed:15169905}.
    分子量
    24.7 kDa Including tag.
    UniProt
    O35716
    途径
    JAK/STAT Signaling, Interferon-gamma Pathway, TLR signaling, Response to Growth Hormone Stimulus
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