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PEMT Protein (AA 2-199) (rho-1D4 tag)

Crystallography grade PEMT 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
产品编号 ABIN3123095
发货至: 中国
  • 抗原 See all PEMT 蛋白
    PEMT (Phosphatidylethanolamine N-Methyltransferase (PEMT))
    蛋白类型
    Recombinant
    产品特性
    AA 2-199
    宿主
    小鼠
    资源
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This PEMT protein is labelled with rho-1D4 tag.
    应用范围
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    序列
    SWLLGYMDPT EPSFVAAVIT IVFNPLFWNV VARWEQRTRK LSRAFGSPHL ACYSLGICIL LLNILRSHCF TQAMMSQPKM EGLDNHTTYF LGLAFLGWGF VFVLSSFYAL GFTGTFLGDY FGILKESRVT TFPFSVLDNP MYWGSTANYL GWALMHASPT GLLLTVLVAI VYVVALLYEE PFTAEIYRQK ATRLHKRS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Pemt Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin-free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product PEMT 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    PEMT (Phosphatidylethanolamine N-Methyltransferase (PEMT))
    别名
    Pemt (PEMT 产品)
    别名
    zgc:55479 Protein, DDBDRAFT_0204815 Protein, DDBDRAFT_0267053 Protein, DDB_0204815 Protein, DDB_0267053 Protein, DDBDRAFT_0219474 Protein, DDBDRAFT_0267052 Protein, DDB_0219474 Protein, DDB_0267052 Protein, PEAMT Protein, PEMPT Protein, PEMT2 Protein, PNMT Protein, AI255394 Protein, Pempt Protein, Pempt2 Protein, PHOMETH Protein, phosphatidylethanolamine N-methyltransferase Protein, pemt Protein, MCA3065 Protein, pmtA Protein, CNG04250 Protein, pemtA Protein, pemtB Protein, PEMT Protein, Pemt Protein
    背景
    Catalyzes three sequential methylation reactions of phosphatidylethanolamine (PE) by AdoMet, thereby producing phosphatidylcholine (PC).
    分子量
    23.6 kDa Including tag.
    UniProt
    Q61907
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