AIF Protein (AA 102-612) (His tag)
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- 抗原 See all AIF (AIFM1) 蛋白
- AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 102-612
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This AIF protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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GLSPEEKQRR AIASATEGGS VPQIRAPSHV PFLLIGGGTA AFAAARSIRA RDPGARVLIV SEDPELPYMR PPLSKELWFS DDPNVTKTLQ FRQWNGKERS IYFQPPSFYV SAQDLPNIEN GGVAVLTGKK VVHLDVRGNM VKLNDGSQIT FEKCLIATGG TPRSLSAIDR AGAEVKSRTT LFRKIGDFRA LEKISREVKS ITVIGGGFLG SELACALGRK SQASGIEVIQ LFPEKGNMGK ILPQYLSNWT MEKVKREGVK VMPNAIVQSV GVSGGRLLIK LKDGRKVETD HIVTAVGLEP NVELAKTGGL EIDSDFGGFR VNAELQARSN IWVAGDAACF YDIKLGRRRV EHHDHAVVSG RLAGENMTGA AKPYWHQSMF WSDLGPDVGY EAIGLVDSSL PTVGVFAKAT AQDNPKSATE QSGTGIRSES ETESEASEIT IPPSAPAVPQ VPVEGEDYGK GVIFYLRDKV VVGIVLWNVF NRMPIARKII KDGEQHEDLN EVAKLFNIHE D
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Aifm1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product AIFM1 蛋白
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Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 420-529) protein (GST tag)
AIFM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 98-609) protein (His tag)AIFM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 1-613) protein (GST tag)AIFM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 102-613) protein (His tag)Crystallography grade AIFM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (Transcript Variant 4) protein (His tag)AIFM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
- 别名
- Aifm1 (AIFM1 产品)
- 别名
- AIF Protein, CMTX4 Protein, COWCK Protein, COXPD6 Protein, PDCD8 Protein, CG7263 Protein, DmAIF Protein, Dmel\\CG7263 Protein, GB16024 Protein, DDBDRAFT_0187853 Protein, DDBDRAFT_0191137 Protein, DDB_0187853 Protein, DDB_0191137 Protein, aif Protein, pdcd8 Protein, AIFM1 Protein, PCD8 Protein, AIFsh2 Protein, Hq Protein, Pdcd8 Protein, mAIF Protein, Aif Protein, zgc:91994 Protein, apoptosis inducing factor mitochondria associated 1 Protein, allograft inflammatory factor 1 Protein, Apoptosis inducing factor Protein, apoptosis-inducing factor 1, mitochondrial Protein, apoptosis inducing factor Protein, apoptosis inducing factor, mitochondria associated 1 Protein, apoptosis-inducing factor, mitochondrion-associated, 1 Protein, apoptosis-inducing factor, mitochondrion-associated 1 Protein, AIFM1 Protein, AIF1 Protein, AIF Protein, LOC412212 Protein, aif Protein, aifm1 Protein, Aifm1 Protein
- 背景
- Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis,and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity). {ECO:0000250, ECO:0000269|PubMed:9989411}.
- 分子量
- 56.5 kDa Including tag.
- UniProt
- Q9Z0X1
- 途径
- Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect
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