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NCKIPSD Protein (AA 1-714) (His tag)

Crystallography grade NCKIPSD 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
产品编号 ABIN3137230
发货至: 中国
  • 抗原 See all NCKIPSD 蛋白
    NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))
    蛋白类型
    Recombinant
    产品特性
    AA 1-714
    宿主
    小鼠
    资源
    • 2
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This NCKIPSD protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    序列
    MYRALYAFRS AEPNAMAFAA GETFLVLERS STHWWLAARA RSGETGYVPP AYLHRLQGME QDVLQAIDRA IEAVHNTAMR DGGKYSLEQR GVLQKLIHHR KETLSRRGTS ASSATVMTPS TSDHHLDAAV SRQPNGVCRT GFERQHSLPS SEHLGTDGAL YQVPPQPRRA APTTPPPPVK RRDREALVIS GSGGRTAIPS GGSSVSSGSS ASSTSMDTLY TGSSPSELGP SCSPTPPPVP RRGAHTTVSQ PQPSPSKAPS PEPPTEEVAA ETNSTPDDLE AQDALSPETT EEKAAAETVV PRTIGAELME LVRRNTGLSH ELCRVAIGVV VGHIQATVPA SSPIMEQVLL SLVEGKDLST ALPSGQVCHD QQRLEVIFAD LARRKDDAQQ RSWALYEDED VIRCYLEELL HILTDADPEV CKKMCKRSDF ESVLALVAYY QMEHRASLRL LLLKCFGAMC SLDAAIISTL VSSVLPVELA RDMQTDTQDH QKLCYSALVL AMVFSMGEAV PYAHYEHLGT PFAQFLLSIV EDGLPMDTTE QLPDLCMNLL LALNLHLTAP EQNVIMAALS RHTNVKIFSE KLLLLLNRGD DPVRIFRHEP QPPHSVLKFL QDVFSSSATA AIFYHTDMMA LIDITVRQIA DLSPGDKLRM EYLSLMHAVV RSTPYLQHRH RLSDLQATLR RILTEEEASP QCQMDRMIVQ EMYKEFPDLG EVPS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Nckipsd Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product NCKIPSD 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))
    别名
    Nckipsd (NCKIPSD 产品)
    别名
    AF3P21 Protein, DIP Protein, DIP1 Protein, ORF1 Protein, SPIN90 Protein, VIP54 Protein, WASLBP Protein, WISH Protein, Wasbp Protein, SH3 adapter protein SPIN90 Protein, NCK interacting protein with SH3 domain Protein, CpipJ_CPIJ019280 Protein, NCKIPSD Protein, Nckipsd Protein
    背景
    Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1) (By similarity). Induces microspike formation, in vivo. In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42. May play an important role in the maintenance of sarcomere and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. {ECO:0000250}.
    分子量
    79.5 kDa Including tag.
    UniProt
    Q9ESJ4
    途径
    Protein targeting to Nucleus
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