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DCP2 Protein (AA 1-422) (His tag)

Crystallography grade DCP2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
产品编号 ABIN3137057
发货至: 中国
  • 抗原 See all DCP2 蛋白
    DCP2 (DCP2 Decapping Enzyme Homolog (DCP2))
    蛋白类型
    Recombinant
    产品特性
    AA 1-422
    宿主
    • 4
    • 1
    小鼠
    资源
    • 2
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This DCP2 protein is labelled with His tag.
    应用范围
    SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
    序列
    MEPKRLEIPG SVLDDLCSRF ILHIPSEERD NAIRVCFQIE LAHWFYLDFY MQNTPGLPQC GIRDFAKAVF SHCPFLLPQG EDVEKILDEW KEYKMGVPTY GAIILDETLE NVLLVQGYLA KSGWGFPKGK VNKEEAPHDC AAREVFEETG FDIKDYICKD DYIELRINDQ LARLYIIPGV PKDTKFNPKT RREIRNIEWF SIEKLPCHRN DMTPKSKLGL APNKFFMAIP FIRPLRDWLS RRFGDSSDSD NGFSSAGSTP ARPTVEKLSR TKFRHSQQLF PEGSPSDQWV KHRQPLQQKS HSNHGEVSDL LKAKNQNMRG NGRKQYQDSP NQKKRANGVH GQPAKQQNPL VKCEKKLHPR KLQDNFETDA TCDLPCSGEE PSVEHAEGHS VACNGHCKFP FSSRAFLSFK FDQNAIMKIL DL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Dcp2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product DCP2 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    DCP2 (DCP2 Decapping Enzyme Homolog (DCP2))
    别名
    Dcp2 (DCP2 产品)
    别名
    DDBDRAFT_0185453 Protein, DDBDRAFT_0252811 Protein, DDB_0185453 Protein, DDB_0252811 Protein, 2410015D23Rik Protein, 5730537H01Rik Protein, AL118268 Protein, NUDT20 Protein, RGD1562909 Protein, mRNA-decapping enzyme 2 Protein, hypothetical protein Protein, decapping mRNA 2 Protein, CpipJ_CPIJ019463 Protein, PTRG_05936 Protein, dcp2 Protein, MCYG_05106 Protein, MGYG_07617 Protein, PGTG_03310 Protein, Tsp_04018 Protein, DCP2 Protein, Dcp2 Protein
    背景
    Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed:21070968). Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed:21070968). Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (By similarity). Plays a role in replication-dependent histone mRNA degradation (By similarity). Has higher activity towards mRNAs that lack a poly(A) tail (PubMed:21070968). Has no activity towards a cap structure lacking an RNA moiety (PubMed:21070968). {ECO:0000250|UniProtKB:Q8IU60, ECO:0000269|PubMed:21070968}.
    分子量
    49.3 kDa Including tag.
    UniProt
    Q9CYC6
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