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Oligophrenin 1 Protein (OPHN1) (AA 1-802) (His tag)

Crystallography grade OPHN1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
产品编号 ABIN3136919
发货至: 中国
  • 抗原 See all Oligophrenin 1 (OPHN1) 蛋白
    Oligophrenin 1 (OPHN1)
    蛋白类型
    Recombinant
    产品特性
    AA 1-802
    宿主
    • 4
    • 1
    • 1
    小鼠
    资源
    • 2
    • 2
    • 1
    • 1
    Insect Cells
    标记
    This Oligophrenin 1 protein is labelled with His tag.
    应用范围
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    序列
    MGHPPLEFSD CYLDSPDFRQ RLKYYEEELE RTNKFIKDVI KDGSALISAM RNYSSAVQKF SQTLQSFQFD FIGDTLTDDE INIAESFKEF AELLNEVENE RMMMVQNASD LLIKPLETFR KEQIGFTKER KKKFEKDGER FYSLLDRHLH LSSKKKESQL LEADLQVDKE RHNFFESSLD YVYQIQEVQE SKKFNIVEPV LAFLHSLFIS NSLTVELTQD FLPYKQQLQL SLQNTRNHFS STREEMEELK KRMKEAPQTC KLPGQPTIEG YLYTQEKWAL GISWAKYYCR YEKETRMLTM IPMEQKPGAK QGPVDLTLKY CVRRKTESID KRFCFDIETN ERPGTITLQA PSEANRRLWM EAMDGKEPIY HTPITKQEEM ELNEVGFKFV RKCINFIETK GIKTEGLYRT VGSNIQVQKL LYAFFDPKCP GDVDFHNSDW DIKTITSSLK FYLRNLSEPV MTYKLHKELV SAAKSDNLDY RLGAIHSLVY KLPEKNREML ELLIKHLVNV CEHSKENLMT PSNMGVIFGP TLMRAQEDTV AAMMNIKFQN IVVEILIEHF GKIYLGPPED SQVPPVPPPR VTARRHKPIT ISKRLLREKT VFYTSSLDEN KDESHHQTPN GTITSNLDPP KLLQHLKPPM QKSGETDPGR KSPSRPVSDC QSEPCLETDV GRLLFRLQDG GTKATPKASN GPVPGSGHTK TSSFHIRRPA PRPMAHHKEG DTDGFSKVRP PGEKQTIIRP PVRPPDPPCR SITPQKPEPK PETGSGNADE IPSSVVASRT RFFETASRKT GSSQGKLPGD ES
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ophn1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product OPHN1 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    Oligophrenin 1 (OPHN1)
    别名
    Ophn1 (OPHN1 产品)
    别名
    ARHGAP41 Protein, MRX60 Protein, OPN1 Protein, C130037N19Rik Protein, Opn1 Protein, MGC68856 Protein, zgc:92603 Protein, OPHN1 Protein, mrx60 Protein, opn1 Protein, oligophrenin 1 Protein, oligophrenin 1 L homeolog Protein, OPHN1 Protein, Ophn1 Protein, ophn1.L Protein, ophn1 Protein, Tsp_13482 Protein
    背景
    Stimulates GTP hydrolysis of members of the Rho family. Its action on RHOA activity and signaling is implicated in growth and stabilization of dendritic spines, and therefore in synaptic function. Critical for the stabilization of AMPA receptors at postsynaptic sites. Critical for the regulation of synaptic vesicle endocytosis at presynaptic terminals (By similarity). Required for the localization of NR1D1 to dendrites, can suppress its repressor activity and protect it from proteasomal degradation. {ECO:0000250, ECO:0000269|PubMed:21874017}.
    分子量
    92.9 kDa Including tag.
    UniProt
    Q99J31
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