EGLN1 Protein (AA 2-400) (His tag)
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- 抗原 See all EGLN1 蛋白
- EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-400
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This EGLN1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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ASDSGGPGVL SASERDRQYC ELCGKMENLL RCGRCRSSFY CCKEHQRQDW KKHKLVCQGG EAPRAQPAPA QPRVAPPPGG APGAARAGGA ARRGDSAAAS RVPGPEDAAQ ARSGPGPAEP GSEDPPLSRS PGPERASLCP AGGGPGEALS PGGGLRPNGQ TKPLPALKLA LEYIVPCMNK HGICVVDDFL GRETGQQIGD EVRALHDTGK FTDGQLVSQK SDSSKDIRGD QITWIEGKEP GCETIGLLMS SMDDLIRHCS GKLGNYRING RTKAMVACYP GNGTGYVRHV DNPNGDGRCV TCIYYLNKDW DAKVSGGILR IFPEGKAQFA DIEPKFDRLL FFWSDRRNPH EVQPAYATRY AITVWYFDAD ERARAKVKYL TGEKGVRVEL KPNSVSKDV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Egln1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product EGLN1 蛋白
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Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1) (AA 272-355) protein (GST tag)
EGLN1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1) (AA 1-128) protein (GST tag)EGLN1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1) (AA 2-426) protein (His tag)Crystallography grade EGLN1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1) protein (Myc-DYKDDDDK Tag)EGLN1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))
- 别名
- Egln1 (EGLN1 产品)
- 别名
- EGLN1 Protein, egln2 Protein, phd2 Protein, zgc:92314 Protein, AI503754 Protein, C1orf12 Protein, HIF-PH2 Protein, HPH-2 Protein, Hif-p4h-2 Protein, ORF13 Protein, Phd2 Protein, SM-20 Protein, PHD-2 Protein, PHD2 Protein, ECYT3 Protein, HIFPH2 Protein, HPH2 Protein, SM20 Protein, ZMYND6 Protein, egl nine homolog 1 (C. elegans) Protein, egl-9 family hypoxia inducible factor 1 Protein, egl-9 family hypoxia-inducible factor 1 S homeolog Protein, egl-9 family hypoxia-inducible factor 1b Protein, egl-9 family hypoxia-inducible factor 1 Protein, EGLN1 Protein, egln1.S Protein, egln1b Protein, Egln1 Protein
- 背景
- Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif. {ECO:0000269|PubMed:18096761, ECO:0000269|PubMed:18500250, ECO:0000269|PubMed:21435465}.
- 分子量
- 43.9 kDa Including tag.
- UniProt
- Q91YE3
- 途径
- cAMP Metabolic Process, Warburg Effect
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