JNK Protein (AA 1-384) (Strep Tag)
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- 抗原 See all JNK (MAPK8) 蛋白
- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-384
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This JNK protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MSRSKRDNNF YSVEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAYDAILER NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE FQDVYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM GYKENVDLWS VGCIMGEMVC HKILFPGRDY IDQWNKVIEQ LGTPCPEFMK KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE EWKELIYKEV MDLEERTKNG VIRGQPSPLA QVQQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product MAPK8 蛋白
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Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-427) protein (GST tag)
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Mitogen-Activated Protein Kinase 8 (MAPK8) (Lys55Met-Mutant) (Active) protein (GST tag)MAPK8 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-384) (Active) proteinMAPK8 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 2-383) protein (His tag)MAPK8 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-427) protein (His tag)MAPK8 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-384) protein (GST tag)MAPK8 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 318-427) protein (GST tag)MAPK8 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-427) protein (Strep Tag)Crystallography grade MAPK8 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
- 别名
- Mapk8 (MAPK8 产品)
- 别名
- JNK Protein, JNK-46 Protein, JNK1 Protein, JNK1A2 Protein, JNK21B1/2 Protein, PRKM8 Protein, SAPK1 Protein, SAPK1c Protein, AI849689 Protein, Prkm8 Protein, BSK Protein, BSK/DJNK Protein, Bsk Protein, CG5680 Protein, D-JNK Protein, D-junk Protein, DBSK/JNK Protein, DJNK Protein, DJNK/bsk Protein, Dmel\\CG5680 Protein, JNK/SAPK Protein, Jnk Protein, Junk Protein, SAPKa Protein, c-Jun Protein, dJNK Protein, jnk Protein, T10F20.15 Protein, jnk1 Protein, sapk1 Protein, mapk8 Protein, zgc:112379 Protein, JNKb Protein, mapk8b Protein, mitogen-activated protein kinase 8 Protein, Stress-activated protein kinase jnk-1 Protein, basket Protein, Protein kinase superfamily protein Protein, mitogen-activated protein kinase 8 L homeolog Protein, mitogen-activated protein kinase 8b Protein, mitogen-activated protein kinase 8B-like Protein, MAPK8 Protein, jnk-1 Protein, Mapk8 Protein, bsk Protein, ATMPK8 Protein, mapk8.L Protein, mapk8b Protein, LOC109094263 Protein
- 背景
- Mitogen-activated protein kinase 8 (MAP kinase 8) (MAPK 8) (EC 2.7.11.24) (Stress-activated protein kinase JNK1) (c-Jun N-terminal kinase 1),FUNCTION: Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death (PubMed:9393873, PubMed:28943315). Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed:11602244). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells (PubMed:10811224). Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (By similarity). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed:36812915). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (PubMed:21297631). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (PubMed:21297631). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (By similarity). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (PubMed:29153991). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (By similarity). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (By similarity). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (By similarity). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed:28943315). {ECO:0000250|UniProtKB:P45983, ECO:0000250|UniProtKB:P49185, ECO:0000269|PubMed:10811224, ECO:0000269|PubMed:11602244, ECO:0000269|PubMed:19966288, ECO:0000269|PubMed:21297631, ECO:0000269|PubMed:22441692, ECO:0000269|PubMed:28943315, ECO:0000269|PubMed:29153991, ECO:0000269|PubMed:36812915, ECO:0000269|PubMed:9393873}.
- 分子量
- 44.2 kDa
- UniProt
- Q91Y86
- 途径
- MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins
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