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REST Protein (AA 1-1082) (His tag)

Crystallography grade REST 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, Crys, ELISA, SDS
产品编号 ABIN3136764
发货至: 中国
  • 抗原 See all REST 蛋白
    REST (RE1-Silencing Transcription Factor (REST))
    蛋白类型
    Recombinant
    产品特性
    AA 1-1082
    宿主
    • 2
    • 1
    • 1
    小鼠
    资源
    • 1
    • 1
    Insect Cells
    标记
    This REST protein is labelled with His tag.
    应用范围
    Western Blotting (WB), Crystallization (Crys), ELISA, SDS-PAGE (SDS)
    序列
    MATQVMGQSS GGGSLFNNSA NMGMALTNDM YDLHELSKAE LAAPQLIMLA NVALTGEASG SCCDYLVGEE RQMAELMPVG DNHFSESEGE GLEESADLKG LENMELGSLE LSAVEPQPVF EASAAPEIYS ANKDPAPETP VAEDKCRSSK AKPFRCKPCQ YEAESEEQFV HHIRIHSAKK FFVEESAEKQ AKAWESGSSP AEEGEFSKGP IRCDRCGYNT NRYDHYMAHL KHHLRAGENE RIYKCIICTY TTVSEYHWRK HLRNHFPRKV YTCSKCNYFS DRKNNYVQHV RTHTGERPYK CELCPYSSSQ KTHLTRHMRT HSGEKPFKCD QCNYVASNQH EVTRHARQVH NGPKPLNCPH CDYKTADRSN FKKHVELHVN PRQFNCPVCD YAASKKCNLQ YHFKSKHPTC PSKTMDVSKV KLKKTKKREA DLLNNAVSNE KMENEQTKTK GDVSGKKNEK PVKAVGKDAS KEKKPGSSVS VVQVTTRTRK SAVAAETKAA EVKHTDGQTG NNPEKPCKAK KNKRKKDAEA HPSEEPVNEG PVTKKKKKSE CKSKIGTNVP KGGGRAEERP GVKKQSASLK KGTKKTPPKT KTSKKGGKLA PKGMGQTEPS SGALAQVGVS PDPALIQAEV TGSGSSQTEL PSPMDIAKSE PAQMEVSLTG PPPVEPAQME PSPAKPPQVE APTYPQPPQR GPAPPTGPAP PTGPAPPTEP APPTGLAEME PSPTEPSQKE PPPSMEPPCP EELPQAEPPP MEDCQKELPS PVEPAQIEVA QTAPTQVQEE PPPVSEPPRV KPTKRSSLRK DRAEKELSLL SEMARQEQVL MGVGLVPVRD SKLLKGNKSA QDPPAPPSPS PKGNSREETP KDQEMVSDGE GTIVFPLKKG GPEEAGESPA ELAALKESAR VSSSEQNSAM PEGGASHSKC QTGSSGLCDV DTEQKTDTVP MKDSAAEPVS PPTPTVDRDA GSPAVVASPP ITLAENESQE IDEDEGIHSH DGSDLSDNMS EGSDDSGLHG ARPTPPEATS KNGKAGLAGK VTEGEFVCIF CDRSFRKEKD YSKHLNRHLV NVYFLEEAAE EQEEQEEREE QE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Rest Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product REST 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    REST (RE1-Silencing Transcription Factor (REST))
    别名
    Rest (REST 产品)
    别名
    NRSF Protein, XBR Protein, 2610008J04Rik Protein, AA407358 Protein, xbr Protein, nrsf Protein, rest Protein, resta Protein, REST Protein, RE1 silencing transcription factor Protein, RE1-silencing transcription factor Protein, RE1-silencing transcription factor S homeolog Protein, REST Protein, Rest Protein, rest.S Protein, rest Protein, LOC615792 Protein
    背景
    Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression (By similarity). {ECO:0000250}.
    分子量
    118.7 kDa Including tag.
    UniProt
    Q8VIG1
    途径
    Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity
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