电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

DPPA3 Protein (AA 1-150) (Strep Tag)

Crystallography grade DPPA3 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
产品编号 ABIN3136626
发货至: 中国
  • 抗原 See all DPPA3 蛋白
    DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))
    蛋白类型
    Recombinant
    产品特性
    AA 1-150
    宿主
    • 2
    • 1
    小鼠
    资源
    • 2
    • 1
    Tobacco (Nicotiana tabacum)
    标记
    This DPPA3 protein is labelled with Strep Tag.
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB), ELISA
    序列
    MEEPSEKVDP MKDPETPQKK DEEDALDDTD VLQPETLVKV MKKLTLNPGV KRSARRRSLR NRIAAVPVEN KSEKIRREVQ SAFPKRRVRT LLSVLKDPIA KMRRLVRIEQ RQKRLEGNEF ERDSEPFRCL CTFCHYQRWD PSENAKIGKN
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    内毒素水平
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))
    别名
    Dppa3 (DPPA3 产品)
    别名
    STELLA Protein, 2410075G02Rik Protein, PCG7 Protein, PGC7 Protein, Stella Protein, developmental pluripotency associated 3 Protein, developmental pluripotency-associated 3 Protein, DPPA3 Protein, Dppa3 Protein
    背景
    Developmental pluripotency-associated protein 3 (Compaction-associated protein 1) (Primordial germ cell protein 7) (Stella),FUNCTION: Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis. {ECO:0000269|PubMed:12124616, ECO:0000269|PubMed:12620990, ECO:0000269|PubMed:14654002, ECO:0000269|PubMed:17143267, ECO:0000269|PubMed:21407207, ECO:0000269|PubMed:22034526, ECO:0000269|PubMed:22722204}.
    分子量
    17.7 kDa
    UniProt
    Q8QZY3
You are here:
客服