OBFC1 Protein (AA 2-378) (His tag)
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- 抗原 See all OBFC1 蛋白
- OBFC1 (Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-378
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This OBFC1 protein is labelled with His tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- 序列
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PQPCLLMECE SSPREEEIPP LFWGLDPVFL AFAKLYIKDI LEMKESQQVP GTYFYNGHPI RRVDIMGAVI SVKERETFYS YGVDDATGVI NCVCWKKLSN AESSSDPAIL STARELSMTS QLKKLQETIE QKTRIGIGDI IRVRGSVRMF REEREICANI YYKVDDPVWN MQIARMLELP KLYQKVYDQP FRNPALQEEE ALNNKDNLDL AGLTSLLSEK IKEFLQEKKM QSFYQQELET VESLQSLASR PVTHSTGSDQ VELKDSGTSG VAQRVFKNAL QLLQEKGLVF QRDSGSDKLY YVTTKDKDLQ QKIYHIIKED CQKPNHMEKG CHLLHILNCV HLNLRWDLSK AVLQRVLELL EDQSDIVSTA DHYYAAF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Obfc1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product OBFC1 蛋白
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Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1) (AA 1-368) protein (His tag)
OBFC1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1) (AA 1-368) protein (GST tag)OBFC1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1) (AA 1-368) protein (Strep Tag)Crystallography grade OBFC1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1) protein (Myc-DYKDDDDK Tag)OBFC1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- OBFC1 (Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1))
- 别名
- Obfc1 (OBFC1 产品)
- 别名
- AAF-44 Protein, AAF44 Protein, RP11-541N10.2 Protein, RPA-32 Protein, STN1 Protein, bA541N10.2 Protein, 0610009H20Rik Protein, 2310057J23Rik Protein, AI413458 Protein, zgc:64174 Protein, ATSTN1 Protein, F10K1.17 Protein, F10K1_17 Protein, STN1, CST complex subunit Protein, Nucleic acid-binding, OB-fold-like protein Protein, STN1 Protein, Stn1 Protein, stn1 Protein
- 背景
- Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis, it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha (PubMed:22748632). The CST complex facilitates recovery from many forms of exogenous DNA damage, seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins. Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres. Promotes general replication start following replication-fork stalling implicating new origin firing. May be in involved in C-strand fill-in during late S/G2 phase independent of ot its role in telomere duplex replication (By similarity). {ECO:0000250|UniProtKB:Q9H668, ECO:0000269|PubMed:19854130, ECO:0000269|PubMed:22748632}.
- 分子量
- 44.3 kDa Including tag.
- UniProt
- Q8K2X3
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