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PAK7 Protein (AA 1-719) (His tag)

Crystallography grade PAK7 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, Crys, WB
产品编号 ABIN3136334
发货至: 中国
  • 抗原 See all PAK7 蛋白
    PAK7 (P21 Protein (Cdc42/Rac)-Activated Kinase 7 (PAK7))
    蛋白类型
    Recombinant
    产品特性
    AA 1-719
    宿主
    • 6
    • 1
    小鼠
    资源
    • 2
    • 2
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This PAK7 protein is labelled with His tag.
    应用范围
    SDS-PAGE (SDS), ELISA, Crystallization (Crys), Western Blotting (WB)
    序列
    MFGKKKKKIE ISGPSNFEHR VHTGFDPQEQ KFTGLPQQWH SLLADTANRP KPMVDPSCIT PIQLAPMKTI VRGNKSCKET SINGLLEDFD NISVTRSNSL RKESPPTPDQ GAASRIQGHS EENGFITFSQ YSSESDTTAD YTTEKYRDRS LYGDDLDLYY KSSHAAKQNG HAMKMKHGDA YYPEMKSLKT DLAGFPVDYH THLDSLRKSS EYGDLRWDYQ RASSSSPLDY SFQLTPSRTA GTSRCSKESL AYSESDWGPS LDDYDRRPKS SYLHQTSPQP AMRQRSKSGS GLQEPMMPFG ASAFKTHPQG HSYNSYTYPR LSEPTMCIPK VDYDRAQMVF SPPLSGSDTY PRGPTKLPQS QSKAGYSSGS HQYPSGYHKA SLYHHPSLQT SSQYISTASY LSSLSISSST YPPPSWGSSS DQQPSRVSHE QFRAALQLVV SPGDPREYLD NFIKIGEGST GIVCIATEKH TGKQVAVKKM DLRKQQRREL LFNEVVIMRD YHHDNVVDMY NSYLVGDELW VVMEFLEGGA LTDIVTHTRM NEEQIATVCL SVLKALSYLH NQGVIHRDIK SDSILLTSDG RIKLSDFGFC AQVSKEVPKR KSLVGTPYWM APEVISRLPY GTEVDIWSLG IMVIEMIDGE PPYFNEPPLQ AMRRIRDSLP PRVKDLHKVS SMLRGFLDLM LVREPSQRAT AQELLGHPFL KLAGPPSCIV PLMRQYRHH
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Pak7 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product PAK7 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    PAK7 (P21 Protein (Cdc42/Rac)-Activated Kinase 7 (PAK7))
    别名
    Pak7 (PAK7 产品)
    别名
    PAK5 Protein, pak5 Protein, 2900083L08Rik Protein, 6430627N20 Protein, Pak5 Protein, PAK-5 Protein, PAK-7 Protein, p21 (RAC1) activated kinase 5 Protein, p21 (RAC1) activated kinase 5 L homeolog Protein, p21 protein (Cdc42/Rac)-activated kinase 7 Protein, p21 (RAC1) activated kinase 7 Protein, PAK5 Protein, pak5.L Protein, Pak7 Protein
    背景
    Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions (By similarity). {ECO:0000250, ECO:0000269|PubMed:11756552}.
    分子量
    81.9 kDa Including tag.
    UniProt
    Q8C015
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