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IBTK Protein (AA 1-1352) (His tag)

Crystallography grade IBTK 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
产品编号 ABIN3135866
发货至: 中国
  • 抗原 See all IBTK products
    IBTK (Inhibitor of Bruton Agammaglobulinemia Tyrosine Kinase (IBTK))
    蛋白类型
    Recombinant
    产品特性
    AA 1-1352
    宿主
    小鼠
    资源
    • 1
    Insect Cells
    标记
    This IBTK protein is labelled with His tag.
    应用范围
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    序列
    MDAATPDCTS KCRSLKHALD VLSVVTKGSE SQIKSFLARY CYNAATVKDA FGRNAGHLAS SCGKKGVLDW LIEKGVDLLV KDKESGWTAL HRSVFYGHID CVWSLLKHGV SLYMQDKEGL SPLDLLMKDR PTHVVFKDTD PTEVYTWGDN TNFTLGHGSQ NSKHHPELLD LFSRSGVYVK QVVLCKFHSV FLSQKGQVYT CGHGRGGRLG HGDEQTCLVP RLVEGLSGHN CSQVAAAKDH TVVLTDDGCV YTFGLNMFHQ LGIIPPPASC NVPRQIQAKY LKGRTIIGVA AGRFHTVLWT REAVYTLGLN GGQLGHLLDP NGEKCVTTPR QVSALHHKDI AVSLVAASDG ATVCVTTRGD IYLLADYQCK KMATKQLNLK KVLVSGGCME YKVDPEHLTE NGGQKICVLA MDGAGRVFCW RSISSSLKQC RWAYPRQVSI SDIALNRNEI LFVTQDGEGF KGKWFEDKRK NSEKKADILP NLHHSSSDVS CVPDTNSVYE RIRLEKLPFA HRAVSVSTDP SGCNFAILQS DPKTSLYEIP VVSSSSFFEE FGKLLRETDE MDSFHDVTFQ VGNRHFPAHK YILAVRSDFF QKLFLSDGSS LELTDVYQKD EDAAGCHLFV VEKVHPDLFE YLLQFMYTDT CDLLTHGFKP RMIVKRKAED CEGSPDSHLH TVNCHVDDKQ KSAFEVYRSN QAHTLSERQK SKPKSSKKGK GVGDDDPVRM LQSVAKKFGL SNLSSRLEGV RLENEKINVI AKKTGNKLKL SQKKCSFLYD VTMKSVDGKE FSCHKCVLCA RLEYFHSMLS RSWIEASSCA ALEMPIQSEI LKVILDYLYT DEAVVIKESQ NVDFVCSVLV VADQLLITRL KEICEVALTE NLTLKNAAML LEFAALYNAG QLKLSCLQFI GLNMAALLEA RSLDVLSEDV LKDLSIFYRK MIPAMERRVI TPYQDGPDIS SMQVEDGEVF FKEEINMEPN YSETMFKKAK TRAKKKPRKR SDSSGGYTLS DVIQSPPSAG LLKSAKTNSV ESLPELLTSD SEGSYAGVAS PRDLQSPDFT AGFHSDKVEG KAKPYVNGIP PPCTREDVKP WEKSPTTKSA PQFIPSNRVD TAASSSWLAG SCSPVSPPVV DLRTIMETEE NRQKYGAAPK SNLGKIISHG IKLSQKQRKM IALTTKENNS GTNSMEAILT APSKSPKPAN AWAPLHSPLS RSFRDFLLEE KKPVPGYGSG DHVKKVCFKG TENSPALNVA RCSTHGTPGL ESNHVSDFPL LDSPNPWQSS SLAASPAVAP VTFASIVEEE RQQEAALIRS REKPLALIQV EEHAIQDLLV FYEAFGNPEE FVVVERAPQG PLAVPMWNKH GC
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ibtk Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    IBTK (Inhibitor of Bruton Agammaglobulinemia Tyrosine Kinase (IBTK))
    别名
    Ibtk (IBTK 产品)
    别名
    IBTK Protein, fi40c08 Protein, wu:fi40c08 Protein, DKFZp468J085 Protein, BTBD26 Protein, BTKI Protein, RP1-93K22.1 Protein, 5430411K16Rik Protein, AA409502 Protein, mKIAA1417 Protein, inhibitor of Bruton tyrosine kinase Protein, inhibitor of Bruton agammaglobulinemia tyrosine kinase Protein, inhibitor of Bruton tyrosine kinase L homeolog Protein, IBTK Protein, ibtk Protein, Ibtk Protein, ibtk.L Protein
    背景
    Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription (By similarity). {ECO:0000250}.
    分子量
    150.5 kDa Including tag.
    UniProt
    Q6ZPR6
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