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Myosin VI Protein (MYO6) (AA 1-1265) (His tag)

Crystallography grade MYO6 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
产品编号 ABIN3135589
发货至: 中国
  • 抗原 See all Myosin VI (MYO6) 蛋白
    Myosin VI (MYO6)
    蛋白类型
    Recombinant
    产品特性
    AA 1-1265
    宿主
    小鼠
    资源
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This Myosin VI protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    序列
    MEDGKPVWAP HPTDGFQMGN IVDIGPDSLT IEPLNQKGKT FGALINQVFP AEEDSKKDVE DNCSLMYLNE ATLLHNVKVR YSKDRIYTYV ANILIAVNPY FDIPKIYSSD TIKSYQGKSL GTMPPHVFGI ADKAFRDMKV LKMSQSIIVS GESGAGKTEN TKFGSKIPDR ILWTGQDIDD RIVEANPLLE AFGNAKTVRN NNSSRFGKFV EIHFNEKSSV VGGFVSHYLL EKSRICVQGK EERNHHIFYR LCAGASEDIR EKLHLSSPDN FRYLNRGCTR FFANKETDKQ ILQNRKSPEY VKAGSLEGSS IRRPWRFYQD VHSHEKNWFG MMKKNFDLFR VVAGVLHLGN IDLEEAGSTS GGCNLKNKSA PSLEYCAELL GLDQDDLRVS LTTRVMLTTA GGTKGTVIKV PLKVEQANNA RDALAKTVYS HLFDHVVNRV NQCFPFETSS YFIGVLDIAG FEYFEHNSFE QFCINYCNEK LQQFFNERIL KEEQELYQKE GLGVNEVHYV DNQDCIELIE VKLVGILDIL DEENRLPQPS DQHFTSVVHQ KHKDHFRLTI PRKSKLAVHR NLRDDEGFII RQLCRGRVLR RQPQYGGGKN NDALHMSLES LICESRDKFI RALFESSTNN SKDTKQKAGK LSFISVGNKF KTQLNLLLDK LRSTGASFIR CIKPNLKMAS HHFEGAQILS QLQCSGMVSV LDLMQGGFPS RASFHELYNM YKKYMPEKLP RLDPRLFCKP LFKALGLNEV DYKFGLTQVF FRPGKFAEFD QIMKSDPDHL AELVKRVNLW LVCSRWKKVQ WCSLSVIKLK NKIKYRAEAC IKMQKPIRMW LCKRRHNPRI DGLVKVGTLK KRLDKFNEVV SALKDGKPEV NRQIKNLEIS IDALMAKFTS TMMTREQIQK EYDALVKSSE DLLSALQKKK QQEEEAERLR RIQEEMEKER KRREEDEERR RKEEEERRMK LEMEPKRKQE EEERKKREDD EKRIQSEVEA QLARQREEES QQQAVLAQEC RDRELALRIA QNESELISDE AQGDMALRRG PAVQATKAAS GTKKHDLSKW KYAELRDTIN TSCDIELLAA CREEFHRRLK VYHAWKSKNK KRNTETEQRA PKSVTDYDFA PFLNNSPQQN PAAQLPARQQ EIDMKRQQRF FRIPFIRPAD QYKDPQNKKK GWWYAHFDGP WIARQMELHP DKPPILLVAG KDDMEMCELN LEETGLTRKR GAEILPRQFE EIWERCGGIQ YLQSAIESRQ ARPTYATAML QNLLK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Myo6 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product MYO6 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    Myosin VI (MYO6)
    别名
    Myo6 (MYO6 产品)
    别名
    95F Protein, 95F MHC Protein, CG5695 Protein, Dm 95F Protein, Dm95F Protein, Dmel\\CG5695 Protein, Dro95F Protein, JAG Protein, Jaguar Protein, Jar Protein, M6 Protein, MHC95F Protein, MYOVI Protein, Mhc95F Protein, MyoVI Protein, Myosin VI Protein, jag Protein, ms(3)06746 Protein, myosins VI Protein, DFNA22 Protein, DFNB37 Protein, BC029719 Protein, Tlc Protein, sv Protein, RGD1560646 Protein, CMY6 Protein, jaguar Protein, myosin VI Protein, jar Protein, LOC373230 Protein, MYO6 Protein, myo6 Protein, Myo6 Protein
    背景
    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. Required for structural integrity of inner ear hair cells.
    分子量
    147.4 kDa Including tag.
    UniProt
    Q64331
    途径
    Sensory Perception of Sound, Dicarboxylic Acid Transport, Asymmetric Protein Localization
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