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MOCOS Protein (AA 1-862) (His tag)

Crystallography grade MOCOS 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
产品编号 ABIN3134923
发货至: 中国
  • 抗原 See all MOCOS 蛋白
    MOCOS (Molybdenum Cofactor Sulfurase (MOCOS))
    蛋白类型
    Recombinant
    产品特性
    AA 1-862
    宿主
    小鼠
    资源
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This MOCOS protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    序列
    MACGAAERGP EPPAFQRHLE ASTQRLAHGY GLRSMSELRD QEFGRLAGTV YLDHAGATLF PQSQLTNFTK DLMENVYGNP HSQNITSKLT HDTVEQVRYR ILTHFHTTPE DYIVIFTAGS TAALRLVAEA FPWVSRSPEN SGSHFCYLTD NHTSVVGMRK VAAAMSVTSI PVKPEDMWSA EGKDAGACDP DCQLPHLFCY PAQSNFSGTR YPLSWVEEVK SGRRSPVNAP GKWFVLLDAA SYVSTSPLDL SAHQADFIPI SFYKIFGLPT GLGALLVNKH VAPLLRKGYF GGGTAAAYLA GEDFYVPRSS VAERFEDGTI SFLDVIALKH GFDALEHLTG GMVNIQQHTF ALVQYTHSAL SSLRYLNGAP VVRIYSDSEF SSPDVQGPII NFNVLDDGGK IIGYSQVDKM ASLYNIHLRT GCFCNLGACQ RHLGLSDEMV KKHFQAGHVC GDDVDIIDGR PTGSVRISFG YMSTLEDAQA FLRFISTIYL RSPSDQPVPQ ASISDAGALT SKSDCHSPQE GSCTDPSVCN GSYPDTNIMD LHPSLSKASS AQQTPQDKAA GILNGDPGSH IVTNIYLYPI KSCAAFEVTK WPVGSQGLLY DRSWMVVNHN GICMSQKQEP RLCLIQPFID LQQRIMVIKA EGMEPIQVPL EEDGEQTQIC QSRVCADRVN TYDCGENVSR WLSKFLGRLC HLIKQSPHFQ RNARKTPKKG QPPGTTVALS LVNEAQYLLV NTSSILELQR QLNASDEHGK EESFSMKDLI SRFRANIITK GARAFEEEKW DEISIGSLHF QVLGPCHRCQ MICINQQTGQ RNQDVFQTLS ESRGRKVNFG VYLMHSYLDL SSPCFLSVGS EVLPVLKDCG VS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mocos Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product MOCOS 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    MOCOS (Molybdenum Cofactor Sulfurase (MOCOS))
    别名
    Mocos (MOCOS 产品)
    别名
    HMCS Protein, MCS Protein, MOS Protein, 1110018O12Rik Protein, DDBDRAFT_0217046 Protein, DDBDRAFT_0252757 Protein, DDB_0217046 Protein, DDB_0252757 Protein, Og Protein, im:7142948 Protein, mocos Protein, molybdenum cofactor sulfurase Protein, MOCOS Protein, Mocos Protein, AOR_1_1090144 Protein, mocos Protein, MCYG_05594 Protein, VDBG_04857 Protein, MGYG_02684 Protein, TERG_06775 Protein, mal Protein
    背景
    Sulfurates the molybdenum cofactor. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form. {ECO:0000255|HAMAP-Rule:MF_03050}.
    分子量
    96.0 kDa Including tag.
    UniProt
    Q14CH1
    途径
    Response to Water Deprivation, ER-Nucleus Signaling
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