PTPN2 Protein (AA 1-406) (Strep Tag)
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- 抗原 See all PTPN2 蛋白
- PTPN2 (Protein tyrosine Phosphatase, Non-Receptor Type 2 (PTPN2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-406
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This PTPN2 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
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MSATIEREFE ELDAQCRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK LQSTENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTKAVV MLNRTVEKES VKCAQYWPTD DREMVFKETG FSVKLLSEDV KSYYTVHLLQ LENINTGETR TISHFHYTTW PDFGVPESPA SFLNFLFKVR ESGCLTPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGE DVNVKQLLLN MRKYRMGLIQ TPDQLRFSYM AIIEGAKYTK GDSNIQKRWK ELSKEDLSPI CDHSQNRVMV EKYNGKRIGS EDEKLTGLPS KVQDTVEESS ESILRKRIRE DRKATTAQKV QQMKQRLNET ERKRKRWLYW QPILTKMGFV SVILVGALVG WTLLFH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product PTPN2 蛋白
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Protein tyrosine Phosphatase, Non-Receptor Type 2 (PTPN2) (AA 1-387) protein (GST tag)
PTPN2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Protein tyrosine Phosphatase, Non-Receptor Type 2 (PTPN2) protein (His tag)PTPN2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Protein tyrosine Phosphatase, Non-Receptor Type 2 (PTPN2) (AA 2-314) (Active) protein (His tag)PTPN2 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE Active
Protein tyrosine Phosphatase, Non-Receptor Type 2 (PTPN2) (AA 1-415) protein (Strep Tag)Crystallography grade PTPN2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein tyrosine Phosphatase, Non-Receptor Type 2 (PTPN2) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)PTPN2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- PTPN2 (Protein tyrosine Phosphatase, Non-Receptor Type 2 (PTPN2))
- 别名
- Ptpn2 (PTPN2 产品)
- 别名
- PTN2 Protein, PTPT Protein, TC-PTP Protein, TCELLPTP Protein, TCPTP Protein, AI325124 Protein, Ptpt Protein, PTP-1B Protein, cb806 Protein, ptpn2 Protein, ptpn2l Protein, wu:fc10h07 Protein, zgc:55339 Protein, zgc:76973 Protein, hm:zeh1546 Protein, zgc:63623 Protein, ptn2 Protein, ptpt Protein, tc-ptp Protein, tcellptp Protein, tcptp Protein, protein tyrosine phosphatase, non-receptor type 2 Protein, protein tyrosine phosphatase, non-receptor type 2 S homeolog Protein, protein tyrosine phosphatase, non-receptor type 2, b Protein, protein tyrosine phosphatase, non-receptor type 2, a Protein, protein tyrosine phosphatase, non-receptor type 2 L homeolog Protein, PTPN2 Protein, Ptpn2 Protein, ptpn2.S Protein, ptpn2b Protein, ptpn2a Protein, ptpn2.L Protein
- 背景
- Tyrosine-protein phosphatase non-receptor type 2 (Protein-tyrosine phosphatase PTP-2) (EC 3.1.3.48) (MPTP),FUNCTION: Non-receptor type tyrosine-specific phosphatase that dephosphorylates receptor protein tyrosine kinases including INSR, EGFR, CSF1R, PDGFR. Also dephosphorylates non-receptor protein tyrosine kinases like JAK1, JAK2, JAK3, Src family kinases, STAT1, STAT3 and STAT6 either in the nucleus or the cytoplasm. Negatively regulates numerous signaling pathways and biological processes like hematopoiesis, inflammatory response, cell proliferation and differentiation, and glucose homeostasis. Plays a multifaceted and important role in the development of the immune system. Functions in T-cell receptor signaling through dephosphorylation of FYN and LCK to control T-cells differentiation and activation. Dephosphorylates CSF1R, negatively regulating its downstream signaling and macrophage differentiation. Negatively regulates cytokine (IL2/interleukin-2 and interferon)-mediated signaling through dephosphorylation of the cytoplasmic kinases JAK1, JAK3 and their substrate STAT1, that propagate signaling downstream of the cytokine receptors. Also regulates the IL6/interleukin-6 and IL4/interleukin-4 cytokine signaling through dephosphorylation of STAT3 and STAT6 respectively. In addition to the immune system, it is involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth. Activated by the integrin ITGA1/ITGB1, it dephosphorylates EGFR and negatively regulates EGF signaling. Dephosphorylates PDGFRB and negatively regulates platelet-derived growth factor receptor-beta signaling pathway and therefore cell proliferation. Negatively regulates tumor necrosis factor-mediated signaling downstream via MAPK through SRC dephosphorylation. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of the hepatocyte growth factor receptor MET. Also plays an important role in glucose homeostasis. For instance, negatively regulates the insulin receptor signaling pathway through the dephosphorylation of INSR and control gluconeogenesis and liver glucose production through negative regulation of the IL6 signaling pathways. May also bind DNA. {ECO:0000269|PubMed:11498795, ECO:0000269|PubMed:11909529, ECO:0000269|PubMed:12138178, ECO:0000269|PubMed:14966296, ECO:0000269|PubMed:15696169, ECO:0000269|PubMed:16705167, ECO:0000269|PubMed:17210636, ECO:0000269|PubMed:20484139, ECO:0000269|PubMed:22080863, ECO:0000269|PubMed:9271584}.
- 分子量
- 47.4 kDa
- UniProt
- Q06180
- 途径
- EGFR Signaling Pathway, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling
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